Influence of M1 and M2 phenotypes of macrophages and P2X7 receptor on neuroblastoma mestastasis

• Grant number: 18/14993-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: September 01, 2018
• End date: October 31, 2020
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Claudiana Lameu
• Grantee: Carolina Adriane Bento
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 15/19128-2 - Metastasis mechanisms of childhood tumors to bone marrow, AP.JP

Abstract

Cancer is characterized by rapid and uncontrolled growth of abnormal cells and is the second leading cause of death (1 in 6 deaths worldwide), with approximately 8.8 million deaths by 2015. Despite many ongoing research, the cancer still has no cure. Therefore, the search for increasingly effective treatments and the elucidation of the molecular mechanisms involved in its development become extremely necessary. Neuroblastoma is the most common cancer in children, affecting 8% to 10% of children's tumors. High-risk neuroblastoma results in metastasis, and only 20% of patients survive up to 5 years after diagnosis, despite aggressive chemotherapy. It is known that tumors are complex tissues, composed of several cell types that are interrelated and help the development of the tumor, which is called a tumor microenvironment. Among these cell types are the macrophages. The activation or polarization of the macrophages varies according to the microenvironmental stimuli from where they are found, and can give rise to a pro-inflammatory phenotype or an anti-inflammatory phenotype. These phenotypes are named M1 and M2, respectively, and can be distinguished according to the responses that are caused. Studies have shown that the P2X7 receptor, which is highly expressed in macrophages, has a great influence on the polarization of macrophages. It has also been observed the association of both phenotypes to a greater capacity of invasion and, consequently, of metastasis. Thus, this project intends to study how the P2X7 receptor influences the polarization of M1 and M2 macrophages and how both phenotypes contribute to neuroblastoma metastasis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ARNAUD-SAMPAIO, VANESSA F.; RABELO, IZADORA L. A.; BENTO, CAROLINA A.; GLASER, TALITA; BEZERRA, JEAN; COUTINHO-SILVA, ROBSON; ULRICH, HENNING; LAMEU, CLAUDIANA. Using Cytometry for Investigation of Purinergic Signaling in Tumor-Associated Macrophages. Cytometry Part A, v. 97, n. 11, JUL 2020. (17/06132-7, 15/13345-1, 18/07366-4, 18/14993-5, 15/19128-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BENTO, Carolina Adriane. The influence of different macrophages\' phenotypes, and of the P2X7 receptor, on the invasiveness and chemoresistance of neuroblastoma. 2020. Master's Dissertation - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.