Effects of the association cisplatin/green propolis ethanolic extract on A549 cell line: a cell death analysis

• Grant number: 18/17402-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2018
• End date: October 31, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Raquel Alves dos Santos
• Grantee: Loren Monielly Pires
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil
• Associated research grant: 17/04138-8 - Attainment of chemical, analytical, biological, pharmacological and technological studies to fill the gaps on the development of Brazilian propolis sector, AP.TEM

Abstract

Lung cancer is one of the most frequent neoplasms with the highest mortality rates worldwide. Despite the current treatments for this cancer, the survival rates after 5 years still remain very low, which indicates the need for alternatives to treat this disease. Cisplatin is the chemotherapic of primary choice for the treatment of lung cancer, however, its nephro and cardiotoxic effects limit its use. Thus, studies associating the standard chemeterapic for a certain neoplasia with some natural product have emerged as an alternative to reducing adverse reactions and increasing efficacy to the proposed treatment. Considering this information, this project aims to study the effects of combined treatment of cisplatin (CIS) with ethanolic extract of green propolis (EEGP) on the induction of cell death in the cell line A549 derived from lung adenocarcinoma compared to normal fibroblasts GM07492-A. The effects of this combination will be evaluate on cell viability using the trypan blue exclusion test and the release of lactate dehydrogenase, as well as on the induction of cell death (apoptosis and necrosis)