Third-generation cephalosporins-resistance in Escherichia coli isolated from retail lamb meat in São José do Rio Preto, SP

• Grant number: 18/16343-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2018
• End date: October 31, 2019
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Tiago Casella
• Grantee: Luana Perpetua Tobias Deus Ajude
• Host Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil

Abstract

The resistance to third-generation cephalosporins (3GC) is an important public health concern worldwide since they are widely used drugs to treat human infections. Several studies report the transmission of bacteria and resistance genes from food to humans via handling or consumption. Detection of Escherichia coli in food indicates possible fecal contamination, and the presence of 3GC-resistant strains points out the possibility of transmission of resistance genes to the consumers' gut microbiota. Research in antimicrobial resistant bacteria from feedlot-lambs is a neglected field in Brazil, although internal consumption of lamb meat shows an exponential increasing in the country. Thus, this project intends to analyze the presence of 3GC-resistant E. coli in lamb meat. For this, 50 retail lamb meat samples will be acquired in São José do Rio Preto, SP. Twenty-five grams will be put in 225 mL of peptone water supplemented with 4 mg/L of ceftiofur and incubated at 37 °C for 20 hours. The resulted broth will be inoculated in MacConkey agar supplemented with 4 mg/L of ceftiofur. Up to 100 presumptive E. coli colonies will be inoculated in a new agar and then preserved. The isolates will be identified by automated system and submitted to antimicrobial susceptibility test by disk-diffusion. The 3GC-resistance determinants will be investigated by PCR and then sequenced. Isolates will be screened by ERIC-PCR and typed by MLST.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOZI, KATIA SUEMI; DEUS AJUDE, LUANA PERPETUA TOBIAS; BARROSO, MARLON DO VALLE; DA SILVA, CAROLINE RODRIGUES; PEIRO, JULIANA REGINA; MENDES, LUIZ CLAUDIO NOGUEIRA; NOGUEIRA, MARA CORREA LELLES; CASELLA, TIAGO. Potentially Pathogenic Multidrug-Resistant Escherichia coli in Lamb Meat. MICROBIAL DRUG RESISTANCE, JAN 2021. (18/16147-4, 19/16003-5, 18/02691-4, 18/16343-8)

GOZI, KATIA SUEMI; FROES, JULIANA RODRIGUES; TOBIAS DEUS AJUDE, LUANA PERPETUA; DA SILVA, CAROLINE RODRIGUES; BAPTISTA, RAFAELA SPERANZA; PEIRO, JULIANA REGINA; MARINHO, MARCIA; NOGUEIRA MENDES, LUIZ CLAUDIO; LELLES NOGUEIRA, MARA CORREA; CASELLA, TIAGO. Dissemination of Multidrug-Resistant Commensal Escherichia coli in Feedlot Lambs in Southeastern Brazil. FRONTIERS IN MICROBIOLOGY, v. 10, JUN 25 2019. (18/16147-4, 18/02691-4, 18/16343-8)

GOZI, KATIA SUEMI; DEUS AJUDE, LUANA PERPETUA TOBIAS; BARROSO, MARLON DO VALLE; DA SILVA, CAROLINE RODRIGUES; PEIRO, JULIANA REGINA; MENDES, LUIZ CLAUDIO NOGUEIRA; NOGUEIRA, MARA CORREA LELLES; CASELLA, TIAGO. Potentially Pathogenic Multidrug-Resistant Escherichia coli in Lamb Meat. MICROBIAL DRUG RESISTANCE, v. 27, n. 8, p. 1071-1078, AUG 1 2021. (18/02691-4, 18/16147-4, 19/16003-5, 18/16343-8)