Prospecting, heterologous expression, purification and characterization of a xylanase

• Grant number: 18/12885-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: January 01, 2019
• End date: January 19, 2020
• Field of knowledge: Agronomical Sciences - Agronomy - Soil Science
• Principal Investigator: João Martins Pizauro Junior
• Grantee: Gabriella Cavazzini Pavarina
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The enzymes are widely used in industrial processes, especially those of microbial origin, since they present desirable properties for the industrial application. Metagenomics has been used to study the diversity of microorganisms as a strategy for prospecting, enabling access to the collective genome of environmental samples, including non-cultivable microorganisms. The use of enzymes in the process of degradation of lignocellulosic biomass and the promotion of these residues to products of high added-value has potential applicability to several industrial sectors. In this sense, xylanases can be added in saccharification processes, in fruit processing, in paper production and in the production of second generation ethanol. Through the hemicellulose's hydrolysis, the formation of compounds of industrial importance such as xylitol, furfural and xylo-oligosaccharides can also occur. In this sense the present project aims to bioprospect xylanase coding sequences through the sequence-driven strategy, using the metagenomic database of the Laboratory of Biochemistry and Microorganisms and Plants (LBMP), in order to identify a potential candidate for kinetic and functional characterization, capable of degrading lignocellulosic biomass efficiently. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PAVARINA, GABRIELLA CAVAZZINI; DE MACEDO LEMOS, ELIANA GERTRUDES; MONTEIRO LIMA, NATALIA SARMANHO; PIZAURO JR, JOAO MARTINS. Characterization of a new bifunctional endo-1,4-beta-xylanase/esterase found in the rumen metagenome. SCIENTIFIC REPORTS, v. 11, n. 1, MAY 17 2021. (18/12885-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PAVARINA, Gabriella Cavazzini. Bifunctional xylanase-esterase enzyme obtained from the ruminal liquid metagenome. 2020. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.