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Soybean seed treatment with micro and nanofertilizer based on nickel hydroxide and nickel sulfate: evaluation of the BNF efficiency and plant growth

Grant number: 18/13738-1
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): December 01, 2018
Effective date (End): October 31, 2020
Field of knowledge:Agronomical Sciences - Agronomy - Soil Science
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:José Lavres Junior
Grantee:Jessica Bezerra de Oliveira
Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Associated scholarship(s):19/04585-0 - Mapping and chemical speciation of soybean seeds and seedlings treated with nickel sources, BE.EP.MS


Nickel (Ni) is an essential element for the metabolism of plants, being considered a micronutrient. It is a structural component of urease and hydrogenase, which in turn perform nitrogen (N) metabolism in many legume species. The objective of this work is to evaluate the application of Ni sources in the treatment of seed or leaf (in V4), if there are differences in the agronomic efficiency between the sources: 1) Ni hydroxide - micrometric dimension (5 µm), 2) sulfate of Ni and 3) nanometric (20 nm) Ni hydroxide in soybean plants; and if there is an increase in the efficiency of biological N fixation (BNF), with increase in N content in the grains produce. The accumulation (and concentration) of Ni in the grains will be determined by ICP-OES analysis. The spatial distribution of Ni in seeds treated with the sources will be done by micro-fluorescence mapping of X-ray (¼-XRF). The activities of the enzyme urease, nitrogenase, nitrate reductase, N nodulation, fresh nod mass and N accumulation in leaves and grains in the phenological stages R3.5 or R5 will be quantified as well as the evaluation of the biological nitrogen fixation (BNF), using the isotopic technique of natural abundance of 15N (´15N 0). The dry mass of grains, roots and shoot will be evaluated in R7. The cultivated species will be Glycine max, in a greenhouse, grown in sandy soil with low amount of Ni available, making six treatments, varying the sources of Ni, applied in the treatment of seeds - with Co, Mo and Bradyrhizobium japonicum - and via foliar at the phenological stage V4. The experimental design used will be the complete randomized blocks, with three Ni sources and four replicates. The biological nitrogen fixation will be quantified by determining the ureides content, by the nitrogenase activity and by means of the ´15N 0. At the end of the experiment, the plants will be harvested and separated in stem, leaves (limbus and petiole) and roots, to determine the concentrations of Ni and N. Grains will also be harvested at the end of the experiment to determine the productivity and quantity of 15N translocated to soybean seeds.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE OLIVEIRA, JESSICA BEZERRA; RODRIGUES MARQUES, JOAO PAULO; RODAK, BRUNA WURR; GALINDO, FERNANDO SHINTATE; CARR, NATALIA FERNANDES; ALMEIDA, EDUARDO; ARAKI, KOITI; GONCALVES, JOSUE MARTINS; DOS REIS, ANDRE RODRIGUES; VAN DER ENT, ANTONY; et al. Fate of nickel in soybean seeds dressed with different forms of nickel. RHIZOSPHERE, v. 21, p. 10-pg., . (15/05942-0, 19/04585-0, 18/13738-1, 15/19121-8)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
OLIVEIRA, Jessica Bezerra de. Soybean seed treatment and foliar application with nickel sources and Ni-particle sizes: an appraisal of biological nitrogen fixation and plant growth.. 2020. Master's Dissertation - Universidade de São Paulo (USP). Centro de Energia Nuclear na Agricultura (CENA/STB) Piracicaba.

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