Evaluation of a new bleaching agent formulation containing sodium trimetaphosphate on color change, trans-amelodentinium penetration of hydrogen peroxide, cytotoxicity, microhardness and heat generation: in vitro study
The objectives of this study will be to evaluate in vitro the addition of sodium trimetaphosphate (TMP) at a concentration of 0.25% associated with 0.05% sodium fluoride (NaF) and 35% hydrogen peroxide (PH) color change; trans-amelodentinatory penetration; surface and longitudinal microhardness; trans-amelodendinyl cytotoxicity and intrapulpal temperature. The treatments will be: 1) 35% hydrogen peroxide (PH); 2) PH + 0.05% NaF (PH / F); 3) PH + 0.25% TMP (PH / TMP); 4) PH + 0.05% NaF + 0.25% TMP (PH / F / TMP) and 5) HP Blue 35% (FGM - Dental Products: HP). After the application of the bleaching gel (single time/3 sessions/every 7 days) the color change will be evaluated by quantified reflection spectrophotometry, quantified peroxide penetration, where the specimens will be placed in an artificial pulp chamber (CPA) containing buffer solution of acetate to evaluate the optical density in a spectrophotometer. The initial microhardness, after treatment (final) and longitudinal, will also be determined. For analysis of cytotoxicity, specimens will be adapted in the CPAs, and the bleaching diffusion products + culture medium will be applied to MDPC-23 odontoblast cells in culture for 1 hour. A K-type thermocouple shall be used for the intrapulp temperature. The results shall be submitted to statistical analysis at a significance level of 5%.
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