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Evaluation of a new bleaching agent formulation containing sodium trimetaphosphate on color change, trans-amelodentinium penetration of hydrogen peroxide, cytotoxicity, microhardness and heat generation: in vitro study

Grant number: 18/08769-5
Support type:Scholarships in Brazil - Master
Effective date (Start): January 01, 2019
Effective date (End): February 29, 2020
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Alberto Carlos Botazzo Delbem
Grantee:Sara Tiemi Felipe Akabane
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


The objectives of this study will be to evaluate in vitro the addition of sodium trimetaphosphate (TMP) at a concentration of 0.25% associated with 0.05% sodium fluoride (NaF) and 35% hydrogen peroxide (PH) color change; trans-amelodentinatory penetration; surface and longitudinal microhardness; trans-amelodendinyl cytotoxicity and intrapulpal temperature. The treatments will be: 1) 35% hydrogen peroxide (PH); 2) PH + 0.05% NaF (PH / F); 3) PH + 0.25% TMP (PH / TMP); 4) PH + 0.05% NaF + 0.25% TMP (PH / F / TMP) and 5) HP Blue 35% (FGM - Dental Products: HP). After the application of the bleaching gel (single time/3 sessions/every 7 days) the color change will be evaluated by quantified reflection spectrophotometry, quantified peroxide penetration, where the specimens will be placed in an artificial pulp chamber (CPA) containing buffer solution of acetate to evaluate the optical density in a spectrophotometer. The initial microhardness, after treatment (final) and longitudinal, will also be determined. For analysis of cytotoxicity, specimens will be adapted in the CPAs, and the bleaching diffusion products + culture medium will be applied to MDPC-23 odontoblast cells in culture for 1 hour. A K-type thermocouple shall be used for the intrapulp temperature. The results shall be submitted to statistical analysis at a significance level of 5%.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FELIPE AKABANE, SARA TIEMI; DANELON, MARCELLE; NUNES, GABRIEL PEREIRA; GRUBA, AMANDA SCARPIN; DE SOUZA-COSTA, CARLOS ALBERTO; DE OLIVEIRA DUQUE, CARLA CAROLINE; GALLINARI, MARJORIE DE OLIVEIRA; FRAGA BRISO, ANDRE LUIZ; BOTAZZO DELBEM, ALBERTO CARLOS. Evaluation of the aesthetic effect, enamel microhardness and trans-amelodentinal cytotoxicity of a new bleaching agent for professional use containing trimetaphosphate and fluoride. JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, v. 114, FEB 2021. Web of Science Citations: 0.

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