Development and characterization of scaffolds based on PCL-PGE and DNA applied to tissue engineering for bone regeneration

• Grant number: 18/10237-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: January 01, 2019
• End date: May 31, 2022
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Antonio Claudio Tedesco
• Grantee: Cristiano Ceron Jayme
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The present project will use the combination of different materials to advocate the construction of scaffold using polycaprolactone (PCL) and polyethylene glycol (PEG) combined with a deoxyribonucleic acid (DNA) hydrogel applied as a human fetal osteoblast cellular transport system (hFOB 1.19) ATCC (CRL-11372), used in bone regeneration. In addition, a second strategy of direct application under the scaffolds of mesenchymal stem cell lines derived from human bone marrow (HBMS) ATCC (PCS-500-012) will be used to obtain functional osteoblasts. aroused great interest due to their potential involvement in the tissue repair process. DNA-based hydrogels have the function of improving the cellular interaction with pre-fabricated scaffolds, homogeneously distributing the cells and thus mimicking the complex structure of the tissues in addition to improving the conditions of cellular interaction, limited in PCL-PEG scaffolds . In a previous work, it was demonstrated the potential of DNA application as high biocompatibility material, allowing the application to regenerative medicine. Thus, in the present project, we aim to: (i) optimize, evaluate the interaction conditions of hydrogel-cells and the molecular mechanisms of interaction and adhesion between hFOB cell line 1.19 and scaffold; (ii) to differentiate the lineage of mesenchymal stem cells (HBMS) leading to the formation of osteoblasts under the scaffold and preceding the development of scaffolds with effective clinical and experimental application and the possibility of commercial use of the present product. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

JAYME, CRISTIANO CERON; PIRES, ANANDA FERREIRA; FERNANDES, DANIELA SILVESTRINI; BI, HONG; TEDESCO, ANTONIO CLAUDIO. DNA polymer films used as drug delivery systems to early-stage diagnose and treatment of breast cancer using 3D tumor spheroids as a model. Photodiagnosis and Photodynamic Therapy, v. 37, p. 12-pg., 2022-03-01. (18/10237-1, 13/50181-1)

TEDESCO, ANTONIO CLAUDIO; SILVA, EMANOEL P. O.; JAYME, CRISTIANO C.; PIVA, HENRIQUE L.; FRANCHI, LEONARDO P.. Cholesterol-rich nanoemulsion (LDE) as a novel drug delivery system to diagnose, delineate, and treat human glioblastoma. Materials Science & Engineering C-Materials for Biological Applications, v. 123, APR 2021. (12/25216-3, 18/10237-1, 13/50181-1, 14/11870-9)

CALORI, ITALO RODRIGO; JAYME, CRISTIANO CERON; UENO, LEONARDO TSUYOSHI; CORRETO MACHADO, FRANCISCO BOLIVAR; TEDESCO, ANTONIO CLAUDIO. Theoretical and experimental studies concerning monomer/aggregates equilibrium of zinc phthalocyanine for future photodynamic action. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, v. 214, p. 513-521, MAY 5 2019. (18/10237-1, 13/50181-1)

REZENDE, NAYARA; JAYME, CRISTIANO CERON; BRASSESCO, MARIA SOL; TEDESCO, ANTONIO CLAUDIO; DE OLIVEIRA, HARLEY FRANCISCO. Standardization of a resazurin-based assay for the evaluation of metabolic activity in oral squamous carcinoma and glioblastoma cells. Photodiagnosis and Photodynamic Therapy, v. 26, p. 371-374, JUN 2019. (18/10237-1, 13/50181-1, 14/09421-1)

SOUZA, CARLA; JAYME, CRISTIANO CERON; REZENDE, NAYARA; TEDESCO, ANTONIO CLAUDIO. Synergistic effect of photobiomodulation and phthalocyanine photosensitizer on fibroblast signaling responses in an in vitro three-dimensional microenvironment. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 222, SEP 2021. (13/50181-1, 18/10237-1, 17/01272-5)

FERREIRA PIRES, ANANDA. Development of DNA polymer films as a drug delivery system for the treatment of oral cancer. DRUG DELIVERY AND TRANSLATIONAL RESEARCH, v. 10, n. 6, SI, JUN 2020. (13/50181-1, 18/10237-1)

JAYME, CRISTIANO CERON; REZENDE, NAYARA; FERNANDES, DANIELA S. .; PAULA, LEONARDO B. DE; DE CASTRO, BARBARA GIMENES; TAKAHASHI, LUANDRA APARECIDA U. .; TEDESCO, ANTONIO CLAUDIO. Target selectivity of cholesterol-phosphatidylcholine liposome loaded with phthalocyanine for breast cancer diagnosis and treatment by photodynamic therapy. Photodiagnosis and Photodynamic Therapy, v. 39, p. 6-pg., 2022-07-25. (18/24004-9, 21/02207-8, 18/10237-1, 15/18684-9, 13/50181-1)

JAYME, CRISTIANO CERON; SOUZA, CARLA; FERNANDES, DANIELA SILVESTRINI; TEDESCO, ANTONIO CLAUDIO. Tailoring the growth and proliferation of human dermal fibroblasts by DNA-based polymer films for skin regeneration. Journal of Biomedical Materials Research Part A, v. 109, n. 11, p. 2381-2391, NOV 2021. (17/01272-5, 18/10237-1, 13/50181-1)

CERON JAYME, CRISTIANO; FERREIRA PIRES, ANANDA; TEDESCO, ANTONIO CLAUDIO. Development of DNA polymer films as a drug delivery system for the treatment of oral cancer. DRUG DELIVERY AND TRANSLATIONAL RESEARCH, v. 10, n. 6, p. 14-pg., 2020-06-22. (18/10237-1, 13/50181-1)