The prognostic value and biological role of overexpressed proteins in oral squamous cell carcinoma identified by mass spectrometry-based proteomic

• Grant number: 17/26764-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: February 01, 2019
• End date: January 10, 2021
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Ricardo Della Coletta
• Grantee: Mauricio da Rocha Dourado
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Despite the improvement in the diagnosis and treatment of patients with oral squamous cell carcinoma (OSCC), the 5-year survival rate remains around 50-60%, without significant changes in the last decades. The treatment decision and patient's prognosis relies on clinical parameters (TNM system) and histopathological features, tools that have not been shown to be widely effective. In that sense, the identification, validation, and clinical implementation of new markers that would help in the treatment selection, and occasionally prove to be new therapeutic targets that also aid the prognosis prediction, is needed and urgent. The aim of this project is to select proteins that are promising markers to be used in patients with OSCC. For that, an initial selection was based on proteins lists originated in previous studies from our group, which resulted in the selection of seven potential targets: tumor-associated calcium signal transducer 2 (TACSTD2), copine-1 (CPNE1), serpin family H member 1 (SERPINH1), stress-induced-phosphoprotein 1 (STIP1), proteasome subunit alpha type-6 (PSMA6), septin 9 (SEPT9) e stanniocalcin 2 (STC2). The selected proteins will be firstly validated by immunohistochemistry, where three of the most promising will be chosen for the further functional assays based on the malignant phenotypes of cancer. With this study, we expect to identify proteins that show prognostic value on OSCC and an important role in the tumor progression, which could reflect on improvement in the disease treatment, in the future. It is important to highlight that, until today, none of the OSCC markers described in the literature are used in the clinic because of the lack of strong evidence.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOURADO, MAURICIO ROCHA; MACHADO, RENATO ASSIS; PARANAIBA, LIVIA MARIS RIBEIRO; GONZALEZ-ARRIAGADA, WILFREDO ALEJANDRO; DA SILVA, SABRINA DANIELA; SAWAZAKI-CALONE, IRIS; GRANER, EDGARD; SALO, TUULA; COLETTA, RICARDO D.. Trophoblast cell surface antigen 2 expression predicts outcome in oral squamous cell carcinomas. ORAL DISEASES, MAR 2021. (18/16077-6, 17/26764-8)

DOURADO, MAURICIO R.; MIWA, KAREN Y. M.; HAMADA, GUILHERME B.; PARANAIBA, LIVIA M. R.; SAWAZAKI-CALONE, IRIS; DOMINGUETI, CATHERINE B.; DE OLIVEIRA, CARINE ERVOLINO; FURLAN, EMYLLE C. B.; LONGO, BRUNA C.; ALMANGUSH, ALHADI; et al. Prognostication for oral squamous cell carcinoma patients based on the tumour-stroma ratio and tumour budding. Histopathology, v. 76, n. 6, MAY 2020. (17/26764-8, 18/16077-6, 18/16754-8, 18/17689-5)

DOLENS, EDER DA SILVA; DOURADO, MAURICIO ROCHA; ALMANGUSH, ALHADI; SALO, TUULA A.; GURGEL ROCHA, CLARISSA ARAUJO; DA SILVA, SABRINA DANIELA; BRENNAN, PETER A.; COLETTA, RICARDO D.. The Impact of Histopathological Features on the Prognosis of Oral Squamous Cell Carcinoma: A Comprehensive Review and Meta-Analysis. FRONTIERS IN ONCOLOGY, v. 11, NOV 10 2021. (18/16077-6, 17/26764-8)

DOMINGUETI, CATHERINE BUENO; MIWA, KAREN YUMIE MENDONCA; DOURADO, MAURICIO ROCHA; SAWAZAKI-CALONE, IRIS; SALO, TUULA A.; PARANAIBA, LIVIA MARIS RIBEIRO; COLETTA, RICARDO D.. Prognostication for oral carcinomas based on two histological scoring systems (BD and iBD models). ORAL DISEASES, v. 27, n. 4, SEP 2020. (17/26764-8, 18/16077-6, 18/16754-8)

DOURADO, MAURICIO ROCHA; MACHADO, RENATO ASSIS; PARANAIBA, LIVIA MARIS RIBEIRO; GONZALEZ-ARRIAGADA, WILFREDO ALEJANDRO; DA SILVA, SABRINA DANIELA; SAWAZAKI-CALONE, IRIS; GRANER, EDGARD; SALO, TUULA; COLETTA, RICARDO D.. Trophoblast cell surface antigen 2 expression predicts outcome in oral squamous cell carcinomas. ORAL DISEASES, v. 28, n. 4, p. 9-pg., 2021-03-05. (17/26764-8, 18/16077-6)