Although the prevalence of oral squamous cell carcinoma (OSCC) has increased worldwide in young patients, there is no consensus regarding its etiological factors. Nevertheless, previous studies have proposed that high-risk human papillomavirus (HR-HPV) may be a promoting agent of oral carcinogenesis in this age group. Preliminary results: Laser-capture microdissection followed by liquid chromatography coupled mass spectrometry were carry out in twenty HR-HPV positive and HR-HPV negative OSCC samples from young patients. 1,030 proteins were identified and 26 were differentially expressed and enriched for biological processes. Five from the 26 proteins were selected for further investigation. Aim: To study the biological function of proteins involved in mTOR pathway to elucidare their role in oral carcinogenesis of HPV-related OSCC. Methods: The human OSCC cell lines UPCI-SCC-154 (HVP-positive) and SCC-9 (HVP-negative) will cultured to evaluate the biological impact of these proteins in preclinical model considering: proliferation, adhesion, migration and invasion produced by the overexpression or inactivation, through of Western blot and quantitative real time-polymerase chain reaction analysis; invasion, migration and adhesion assays.
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