Development of labelled nanopesticides aiming studies of mechanism of action and toxicity

• Grant number: 18/23608-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: February 01, 2019
• End date: January 31, 2022
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Leonardo Fernandes Fraceto
• Grantee: Lucas Bragança de Carvalho
• Host Institution: Instituto de Ciência e Tecnologia. Universidade Estadual Paulista (UNESP). Campus de Sorocaba. Sorocaba , SP, Brazil
• Associated research grant: 17/21004-5 - Agriculture, micro/nanotechnology and environment: from evaluation of the mechanisms of action to studies of transport and toxicity, AP.TEM

Abstract

Nanopesticides today represent an emerging technology that is well studied because of its potential benefits. Most nanopesticides are more effective, and degrade more slowly when applied in the environment. In this way, there is a decrease in the general consumption of these compounds, making them more profitable, resulting in stability of crop yield and food safety. In addition, it is worth mentioning that the use of nanopesticides can consequently generate secondary benefits, such as reducing investments in pesticide production as well as reducing environmental pollution. Although many benefits are associated with these systems, the risks associated with pesticides at the nanometer scale are complex, still poorly studied and not fully understood. It is already known that the transport and destination of the nanopesticides presents different when compared to the conventional pesticides. In this sense, the proposal of this project involves the development of different types of nanoparticles composed of synthetic and / or natural polymers for the transport of atrazine. In addition, these nanoparticles will be labeled using different strategies, using fluorescent dyes/probes and hybrid systems with encapsulated gold nanoparticles to enable tracking of these systems in different matrices. The proposal will be divided into three main stages, the first being the functionalization of zein and chitosan with fluorescein isotiocyanate or rhodamy, in the second step will be prepared nanoparticles with this natural polymers marked and development of probe-labeled poly (µ-caprolactone) nanoparticles fluorescers and gold nanoparticles and in the third stage the toxicity of these nanoparticles in vitro will be analyzed through cell and in vivo viability assays by evaluating the development of atrazine and non-target species of corn and mustard plants, respectively. (AU)

Scientific publications

(14)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAMARA, MARCELA CANDIDO; MONTEIRO, RENATA APARECIDA; CARVALHO, LUCAS BRAGANCA; OLIVEIRA, JHONES LUIZ; FRACETO, LEONARDO F.. Enzyme Stimuli-Responsive Nanoparticles for Bioinsecticides: An Emerging Approach for Uses in Crop Protection. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 9, n. 1, p. 106-112, JAN 11 2021. (18/23608-8, 18/21142-1)

GUILGER-CASAGRANDE, MARIANA; GERMANO-COSTA, TAIS; BILESKY-JOSE, NATALIA; PASQUOTO-STIGLIANI, TATIANE; CARVALHO, LUCAS; FRACETO, LEONARDO F.; DE LIMA, RENATA. Influence of the capping of biogenic silver nanoparticles on their toxicity and mechanism of action towards Sclerotinia scierotiorum. JOURNAL OF NANOBIOTECHNOLOGY, v. 19, n. 1, FEB 24 2021. (16/13692-6, 18/23608-8, 17/20932-6, 17/13328-5, 20/05816-2, 17/21004-5)

BILESKY-JOSE, NATALIA; MARUYAMA, CINTIA; GERMANO-COSTA, TAIS; CAMPOS, ESTEFANIA; CARVALHO, LUCAS; GRILLO, RENATO; FRACETO, LEONARDO FERNANDES; DE LIMA, RENATA. Biogenic alpha-Fe2O3 Nanoparticles Enhance the Biological Activity of Trichoderma against the Plant Pathogen Sclerotinia sclerotiorum. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 9, n. 4, p. 1669-1683, FEB 1 2021. (18/23608-8, 17/21004-5, 17/20932-6)

MONTEIRO, RENATA APARECIDA; CAMARA, MARCELA CANDIDO; DE OLIVEIRA, JHONES LUIZ; RAMOS CAMPOS, ESTEFANIA VANGELIE; CARVALHO, LUCAS BRAGANCA; DE FREITAS PROENCA, PATRICIA LUIZA; GUILGER-CASAGRANDE, MARIANA; LIMA, RENATA; DO NASCIMENTO, JOACIR; GONCALVES, KELLY CRISTINA; et al. Zein based-nanoparticles loaded botanical pesticides in pest control: An enzyme stimuli-responsive approach aiming sustainable agriculture. JOURNAL OF HAZARDOUS MATERIALS, v. 417, SEP 5 2021. (17/21004-5, 17/13328-5, 18/21142-1, 18/23608-8, 20/05816-2)

MUJTABA, MUHAMMAD; KHAWAR, KHALID MAHMOOD; CAMARA, MARCELA CANDIDO; CARVALHO, LUCAS BRAGANCA; FRACETO, LEONARDO FERNANDES; MORSI, RANIA E.; ELSABEE, MAHER Z.; KAYA, MURAT; LABIDI, JALEL; ULLAH, HIDAYAT; et al. Chitosan-based delivery systems for plants: A brief overview of recent advances and future directions. International Journal of Biological Macromolecules, v. 154, p. 683-697, JUL 1 2020. (18/23608-8, 17/21004-5)

LAU, ELIZABETH C. H. T.; CARVALHO, LUCAS B.; PEREIRA, ANDERSON E. S.; MONTANHA, GABRIEL S.; CORREA, CAMILA G.; CARVALHO, HUDSON W. P.; GANIN, ALEXEY Y.; FRACETO, LEONARDO F.; YIU, HUMPHREY H. P.. Localization of Coated Iron Oxide (Fe3O4) Nanoparticles on Tomato Seeds and Their Effects on Growth. ACS APPLIED BIO MATERIALS, v. 3, n. 7, p. 4109-4117, JUL 20 2020. (18/02413-4, 18/23608-8, 15/19121-8, 15/05942-0)

PREISLER, ANA CRISTINA; CARVALHO, LUCAS BRAGANCA; SARAIVA-SANTOS, TELMA; VERRI JR, WALDICEU APARECIDO; SAMPAIO MAYER, JULIANA LISCHKA; FRACETO, LEONARDO FERNANDES; DALAZEN, GILIARDI; OLIVEIRA, HALLEY CAIXETA. Interaction of Nanoatrazine and Target Organism: Evaluation of Fate and Photosystem II Inhibition in Hydroponically Grown Mustard (Brassica juncea) Plants. Journal of Agricultural and Food Chemistry, v. 70, n. 25, p. 9-pg., 2022-06-08. (17/21004-5, 18/23608-8)

CARVALHO, LUCAS BRAGANCA; GODOY, ISABELA SILVA; PREISLER, ANA CRISTINA; PROENCA, PATRICIA LUIZA DE FREITAS; SARAIVA-SANTOS, TELMA; VERRI JR, WALDICEU APARECIDO; OLIVEIRA, HALLEY CAIXETA; DALAZEN, GILIARDI; FRACETO, LEONARDO FERNANDES. Pre-emergence herbicidal efficiency and uptake of atrazine-loaded zein nanoparticles: a sustainable alternative to weed control. ENVIRONMENTAL SCIENCE-NANO, v. 10, n. 6, p. 15-pg., 2023-04-21. (18/23608-8, 17/21004-5)

CAMPOS, ESTEFANIA V. R.; PEREIRA, ANDERSON E. S.; DE OLIVEIRA, JHONES LUIZ; CARVALHO, LUCAS BRAGANCA; GUILGER-CASAGRANDE, MARIANA; DE LIMA, RENATA; FRACETO, LEONARDO FERNANDES. How can nanotechnology help to combat COVID-19? Opportunities and urgent need. JOURNAL OF NANOBIOTECHNOLOGY, v. 18, n. 1, SEP 5 2020. (17/21004-5, 19/05100-0, 18/21142-1, 18/23608-8, 20/05816-2, 17/24402-1)

TAKESHITA, VANESSA; DE SOUSA, BRUNO TEIXEIRA; PREISLER, ANA CRISTINA; CARVALHO, LUCAS BRAGANCA; SANTO PEREIRA, ANDERSON DO ESPIRITO; TORNISIELO, VALDEMAR LUIZ; DALAZEN, GILIARDI; OLIVEIRA, HALLEY CAIXETA; FRACETO, LEONARDO FERNANDES. Foliar absorption and field herbicidal studies of atrazine-loaded polymeric nanoparticles. JOURNAL OF HAZARDOUS MATERIALS, v. 418, SEP 15 2021. (19/04758-1, 18/23608-8, 17/21004-5)

CARVALHO, LUCAS BRAGANCA; VENCESLAU, ADNEIA DE FATIMA ABREU; BREISCH, DANIELA LUZ AMBROSIO; FRACETO, LEONARDO FERNANDES; JAIME, CARLOS; PINTO, LUCIANA MATOS ALVES. Heterocyclic agrochemical hosted by cyclodextrin and hybrid cyclodextrin-silica materials: Characterization, release behavior, and mobility in soil. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 656, p. 11-pg., 2023-01-05. (17/21004-5, 18/23608-8)

ANTONACCI, AMINA; FRISULLI, VALERIA; CARVALHO, LUCAS BRAGANCA; FRACETO, LEONARDO FERNANDES; MIRANDA, BRUNO; DE STEFANO, LUCA; JOHANNINGMEIER, UDO; GIARDI, MARIA TERESA; SCOGNAMIGLIO, VIVIANA. An All-Green Photo-Electrochemical Biosensor Using Microalgae Immobilized on Eco-Designed Lignin-Based Screen-Printed Electrodes to Detect Sustainable Nanoherbicides. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 12, p. 12-pg., 2023-06-01. (18/23608-8, 17/21004-5)

PROENCA, PATRICIA L. F.; CARVALHO, LUCAS B.; CAMPOS, ESTEFAENIA V. R.; FRACETO, LEONARDO F.. Fluorescent labeling as a strategy to evaluate uptake and transport of polymeric nanoparticles in plants. ADVANCES IN COLLOID AND INTERFACE SCIENCE, v. 305, p. 15-pg., 2022-07-01. (17/21004-5, 18/23608-8)

MUJTABA, MUHAMMAD; WANG, DEPENG; CARVALHO, LUCAS BRAGANCA; OLIVEIRA, JHONES LUIZ; SANTO PEREIRA, ANDERSON DO ESPIRITO; SHARIF, RAHAT; JOGAIAH, SUDISHA; PAIDI, MURALI KRISHNA; WANG, LICHEN; ALI, QASID; et al. Nanocarrier-Mediated Delivery of miRNA, RNAi, and CRISPR-Cas for Plant Protection: Current Trends and Future Directions. ACS AGRICULTURAL SCIENCE & TECHNOLOGY, v. 1, n. 5, p. 19-pg., 2021-10-18. (17/21004-5, 18/21142-1, 18/23608-8)