Development of microfluidic devices/organ on a chip using xurography, poliester-toner and gelatin methacryloyl (GelMA)

• Grant number: 18/07342-8
• Support Opportunities: Scholarships abroad - Research
• Start date: August 23, 2021
• End date: August 22, 2022
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Rondinelli Donizetti Herculano
• Grantee: Rondinelli Donizetti Herculano
• Host Investigator: Alireza Khademhosseini
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: University of California, Los Angeles (UCLA), United States
• Associated research grant: 16/01743-5 - The new gestational triad: hyperglycemia, urinary incontinence (UI) and biomolecular profile as a long-term predictor for UI: a prospective cohort study: translational research with biodevice with stem cells for muscle regeneration in diabetic rats, AP.TEM

Abstract

Evaluation of cytotoxicity of new drugs, compounds and biomaterials using two-dimensional cell culture is an interesting alternative to reduce the use of animals. However, growing cells in flat layers on plastic surfaces does not accurately model the in vivo state. So, organs-on-chips present an ideal microenvironment to mimic a tissue/organ human-specific. The objective of this work is to develop a low cost microfluidic devices based to polyester-toner technique and xurography using cells embedded in gelatin methacrylamide (GelMA) hydrogels. The excellent biocompatibility of GelMA hydrogels makes them suitable as cell culture matrices that mimic native extracellular matrix (ECM). In addition, we will evaluate the vegetable extracts (Stryphnodendron sp and Casearia sylvestris) and latex membrane incorporated with these extracts and their fractions using these microfluidic chips. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ABDALLA, GABRIELA; MUSSAGY, CASSAMO USSEMANE; BRASIL, GIOVANA SANT'ANA PEGORIN; SCONTRI, MATEUS; SASAKI, JOSANA CARLA DA SILVA; SU, YANJIN; BEBBER, CAMILA; ROCHA, RAILDIS RIBEIRO; ABREU, ANA PAULA DE SOUSA; GONCALVES, ROGERIO PENNA; et al. Eco-sustainable coatings based on chitosan, pectin, and lemon essential oil nanoemulsion and their effect on strawberry preservation. International Journal of Biological Macromolecules, v. 249, p. 15-pg., 2023-09-30. (18/07342-8, 18/21887-7, 17/19603-8, 11/17411-8, 15/11759-3)

HERCULANO, RONDINELLI DONIZETTI; DOS SANTOS, THAINA OLIVEIRA; DE BARROS, NATAN ROBERTO; PEGORIN BRASIL, GIOVANA SANT'ANA; SCONTRI, MATEUS; CARVALHO, BRUNA GREGATTI; MECWAN, MARVIN; FARHADI, NEDA; KAWAKITA, SATORU; PEREGO, CAIO HUMBERTO; et al. Aloe vera-loaded natural rubber latex dressing as a potential complementary treatment for psoriasis. International Journal of Biological Macromolecules, v. 242, p. 13-pg., 2023-05-12. (18/07342-8, 17/19603-8, 21/09207-3)

SILVA, FLAVIO KUNERT DE SOUZA; COSTA-ORLANDI, CAROLINE BARCELOS; FERNANDES, MARIZA AIRES; BRASIL, GIOVANA SANT'ANA PEGORIN; MUSSAGY, CASSAMO USSEMANE; SCONTRI, MATEUS; SASAKI, JOSANA CARLA DA SILVA; ABREU, ANA PAULA DE SOUSA; GUERRA, NAYRIM BRIZUELA; FLORIANO, JULIANA FERREIRA; et al. Biocompatible anti-aging face mask prepared with curcumin and natural rubber with antioxidant properties. International Journal of Biological Macromolecules, v. 242, p. 12-pg., 2023-05-13. (18/07342-8, 17/19603-8, 21/09207-3)

COSTABEBER, GABRIEL; GUERRA, NAYRIM BRIZUELA; BRASIL, GIOVANA SANT 'ANA PEGORIN; SASAKI, JOSANA CARLA DA SILVA; SCONTRI, MATEUS; BURD, BETINA SAYEG; SU, YANJIN; TANAKA, JEAN LUCAS; MANDAL, KALPANA; MECWAN, MARVIN; et al. Additive manufacturing of polylactic acid scaffolds dip-coated with polycaprolactone for bone tissue engineering. MATERIALS TODAY COMMUNICATIONS, v. 40, p. 12-pg., 2024-08-01. (14/17526-8, 11/17411-8, 18/07342-8, 21/09207-3)

MARQUES, PAULO AUGUSTO CHAGAS; GUERRA, NAYRIM BRIZUELA; DOS SANTOS, LINDOMAR SOARES; MUSSAGY, CASSAMO USSEMANE; BRASIL, GIOVANA SANT 'ANA PEGORIN; BURD, BETINA SAYEG; SU, YANJIN; SASAKI, JOSANA CARLA DA SILVA; SCONTRI, MATEUS; LOPES FILHO, PAULO EDUARDO DE LIMA; et al. Natural rubber latex-based biomaterials for drug delivery and regenerative medicine: Trends and directions. International Journal of Biological Macromolecules, v. 267, p. 18-pg., 2024-04-17. (21/09207-3, 18/07342-8)

DE BARROS, NATAN ROBERTO; CHAGAS, ANA LAURA DESTRO; FORSTER, SAMUEL; BRASIL, GIOVANA SANT'ANA PEGORIN; RIBEIRO, GERALDO MONTEIRO; OLIVATO, ORESTES PEREIRA; RAMALHO, LEANDRA NAIRA ZAMBELLI; GUERRA, NAYRIM BRIZUELA; MUSSAGY, CASSAMO USSEMANE; FLORIANO, JULIANA FERREIRA; et al. Novel highly flexible collagen-loaded latex dressing to treat diabetic foot ulcer: A pilot clinical trial. International Journal of Biological Macromolecules, v. 308, p. 20-pg., 2025-05-01. (17/19603-8, 11/17411-8, 23/17448-6, 17/02518-8, 18/07342-8)

HERCULANO, RONDINELLI DONIZETTI; MUSSAGY, CASSAMO USSEMANE; GUERRA, NAYRIM BRIZUELA; BRASIL, GIOVANA SANT'ANA PEGORIN; FLORIANO, JULIANA FERREIRA; BURD, BETINA SAYEG; SU, YANJIN; SASAKI, JOSANA CARLA DA SILVA; MARQUES, PAULO AUGUSTO CHAGAS; SCONTRI, MATEUS; et al. Recent advances and perspectives on natural latex serum and its fractions for biomedical applications. BIOMATERIALS ADVANCES, v. 157, p. 15-pg., 2023-12-27. (21/09207-3, 18/07342-8)

MORAIS, PAMELA CASSIA ROCHA; FLORIANO, JULIANA FERREIRA; GARCIA, CRISTIANE GARCIA PAULINO; CHAGAS, ANA LAURA DESTRO; MUSSAGY, CASSAMO USSEMANE; GUERRA, NAYRIM BRIZUELA; BRASIL, GIOVANA SANT'ANA PEGORIN; VICENTINE, KARINA FERRAZZOLI DEVIENNE; ROCHA, LENALDO BRANCO; OLIVEIRA, CARLO JOSE FREIRE; et al. Comparing the wound healing potential of natural rubber latex serum and F1-protein: An in vivo approach. BIOMATERIALS ADVANCES, v. 157, p. 12-pg., 2024-01-10. (21/09207-3, 18/07342-8)

FORSTER, SAMUEL; FARHADI, NEDA; FILHO, PAULO EDUARDO DE LIMA LOPES; PIAZZA, RODOLFO DEBONE; COSTA-MARQUES, RODRIGO FERNANDO; TANAKA, JEAN LUCAS; SCONTRI, MATEUS; DOS SANTOS, LINDOMAR SOARES; CAI, YI; CAO, WEI; et al. Honey-loaded natural rubber dressing for treatment of atopic dermatitis: A physicochemical, antioxidant, in vitro and in vivo characterization. International Journal of Biological Macromolecules, v. 322, p. 16-pg., 2025-08-12. (15/11759-3, 18/07342-8, 23/17448-6)

GIOVANETTI, KARINA; TUMA, RAFAEL BRULL; BRASIL, GIOVANA SANT'ANA PEGORIN; MIRANDA, MATHEUS CARLOS ROMEIRO; BORGES, FELIPE AZEVEDO; TANAKA, JEAN LUCAS; BURD, BETINA SAYEG; CORTELLAZZI, KARINE LAURA; GUERRA, NAYRIM BRIZUELA; MUSSAGY, CASSAMO USSEMANE; et al. β-Tricalcium phosphate incorporated natural rubber latex membranes for calvarial bone defects: Physicochemical, in vitro and in vivo assessment. International Journal of Biological Macromolecules, v. 282, p. 16-pg., 2024-12-01. (14/17526-8, 18/07342-8, 17/19603-8, 16/02645-7)