Development of microfluidic devices/organ on a chip using xurography, poliester-toner and gelatin methacryloyl (GelMA)

• Grant number: 18/07342-8
• Support Opportunities: Scholarships abroad - Research
• Effective date (Start): August 23, 2021
• Effective date (End): August 22, 2022
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Rondinelli Donizetti Herculano
• Grantee: Rondinelli Donizetti Herculano
• Host Investigator: Alireza Khademhosseini
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Research place: University of California, Los Angeles (UCLA), United States
• Associated research grant: 16/01743-5 - The new gestational triad: hyperglycemia, urinary incontinence (UI) and biomolecular profile as a long-term predictor for UI: a prospective cohort study: translational research with biodevice with stem cells for muscle regeneration in diabetic rats, AP.TEM

Abstract

Evaluation of cytotoxicity of new drugs, compounds and biomaterials using two-dimensional cell culture is an interesting alternative to reduce the use of animals. However, growing cells in flat layers on plastic surfaces does not accurately model the in vivo state. So, organs-on-chips present an ideal microenvironment to mimic a tissue/organ human-specific. The objective of this work is to develop a low cost microfluidic devices based to polyester-toner technique and xurography using cells embedded in gelatin methacrylamide (GelMA) hydrogels. The excellent biocompatibility of GelMA hydrogels makes them suitable as cell culture matrices that mimic native extracellular matrix (ECM). In addition, we will evaluate the vegetable extracts (Stryphnodendron sp and Casearia sylvestris) and latex membrane incorporated with these extracts and their fractions using these microfluidic chips. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HERCULANO, RONDINELLI DONIZETTI; DOS SANTOS, THAINA OLIVEIRA; DE BARROS, NATAN ROBERTO; PEGORIN BRASIL, GIOVANA SANT'ANA; SCONTRI, MATEUS; CARVALHO, BRUNA GREGATTI; MECWAN, MARVIN; FARHADI, NEDA; KAWAKITA, SATORU; PEREGO, CAIO HUMBERTO; et al. Aloe vera-loaded natural rubber latex dressing as a potential complementary treatment for psoriasis. International Journal of Biological Macromolecules, v. 242, p. 13-pg., 2023-05-12. (18/07342-8, 17/19603-8, 21/09207-3)

SILVA, FLAVIO KUNERT DE SOUZA; COSTA-ORLANDI, CAROLINE BARCELOS; FERNANDES, MARIZA AIRES; BRASIL, GIOVANA SANT'ANA PEGORIN; MUSSAGY, CASSAMO USSEMANE; SCONTRI, MATEUS; SASAKI, JOSANA CARLA DA SILVA; ABREU, ANA PAULA DE SOUSA; GUERRA, NAYRIM BRIZUELA; FLORIANO, JULIANA FERREIRA; et al. Biocompatible anti-aging face mask prepared with curcumin and natural rubber with antioxidant properties. International Journal of Biological Macromolecules, v. 242, p. 12-pg., 2023-05-13. (18/07342-8, 17/19603-8, 21/09207-3)

ABDALLA, GABRIELA; MUSSAGY, CASSAMO USSEMANE; BRASIL, GIOVANA SANT'ANA PEGORIN; SCONTRI, MATEUS; SASAKI, JOSANA CARLA DA SILVA; SU, YANJIN; BEBBER, CAMILA; ROCHA, RAILDIS RIBEIRO; ABREU, ANA PAULA DE SOUSA; GONCALVES, ROGERIO PENNA; et al. Eco-sustainable coatings based on chitosan, pectin, and lemon essential oil nanoemulsion and their effect on strawberry preservation. International Journal of Biological Macromolecules, v. 249, p. 15-pg., 2023-09-30. (18/07342-8, 18/21887-7, 17/19603-8, 11/17411-8, 15/11759-3)