Proteomic profile of bacteria present in root canals of teeth with symptomatic or asymptomatic apical periodontitis

• Grant number: 18/08282-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2019
• End date: February 29, 2020
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Rogerio de Castilho Jacinto
• Grantee: Felipe Ricardo Nunes de Moraes
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Endodontic infections are caused by a multi-species community of bacteria..Thus, proteomic analysis is a technique that can revolutionize the study of the pathogenesis of endodontic infections. The present study aims to analyze the proteomic profile of endodontic infections related to teeth with symptomatic and asymptomatic apical periodontitis using liquid chromatography associated with mass spectrometry. The analysis of this proteomic profile aims to provide an understanding of the ecological and pathogenic aspects of the behavior of endodontic bacterial communities through the identification of proteins expressed in said environment at the moment of collection and the determination of the function of these proteins. Samples will be collected from18 patients referred for root canal treatment or emergency treatment at the Araçatuba Dental School FOA - UNESP. Teeth with primary endodontic infection, symptomatic or asymptomatic will be included. The identification of the peptides will be done in a nanoACQUITY UPLC-Xevo QTof MS system(Waters), protein identification will be obtained using ProteinLynx Global Serversoftware (PLGS) version 3.0 using the National Center's bacterial protein databases of Biotechnology Information. The difference of expression betweenthe groups will be obtained through the same software, considering p <0.05 forsubregulated proteins and 1-p> 0.95 for supraregulated proteins. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOUREIRO, C.; BUZALAF, M. A. R.; MORAES, F. R. N.; VENTURA, T. M. O.; PELA, V. T.; PESSAN, J. P.; JACINTO, R. C.. Quantitative proteomic analysis in symptomatic and asymptomatic apical periodontitis. International Endodontic Journal, FEB 2021. (19/14995-0, 18/08282-9, 18/18741-0)

CAROLINE LOUREIRO; MARÍLIA AFONSO RABELO BUZALAF; JULIANO PELIM PESSAN; FELIPE RICARDO NUNES DE MORAES; VINÍCIUS TAIOQUI PELÁ; TALITA MENDES OLIVEIRA VENTURA; ROGÉRIO DE CASTILHO JACINTO. Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study. Brazilian Dental Journal, v. 31, n. 3, p. 319-336, 2020-07-13. (18/08282-9, 18/18741-0, 19/14995-0)

LOUREIRO, C.; BUZALAF, M. A. R.; MORAES, F. R. N.; VENTURA, T. M. O.; PELA, V. T.; PESSAN, J. P.; JACINTO, R. C.. Quantitative proteomic analysis in symptomatic and asymptomatic apical periodontitis. International Endodontic Journal, v. 54, n. 6, p. 14-pg., 2021-02-17. (18/08282-9, 19/14995-0, 18/18741-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MORAES, Felipe Ricardo Nunes de. Proteomic profile of bacteria present in root canals of teeth with symptomatic or asymptomatic apical periodontitis.. 2020. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.