Inflammatory evaluation of lung donor experimental model for transplant

• Grant number: 18/21457-2
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): March 01, 2019
• Effective date (End): December 31, 2019
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal researcher: Natália Aparecida Nepomuceno
• Grantee: Fabiana da Silva Carvalho
• Home Institution: Instituto do Coração Professor Euryclides de Jesus Zerbini (INCOR). Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Experimental Models are important tools to understanding physiological and etiopathogenic issues. Some types of animal are used as experimental models to study elements involved in the pathophysiology of primary graft dysfunction after lung transplantation and to evaluate new treatments. Currently, we can divided experimental model of lung donors into two large groups: donation-after brain death donors and donation after circulatory death donors. In addition, the deleterious effects of hemorrhagic shock and Ischemia and Reperfusion should be considered when we think of donors models. In this study we aim to describe and compare related with four types of lung donors study models: Donation after Brain Death; Donation after Circulatory Death, Hemorrhagic Shock and Cold Ischemia. A number of 50 rats will divided into 5 groups: Control (n=10); Hemorrhagic Shock (n=10); Brain Death (n=10); Circulatory Death (n=10); Cold Ischemia (n=10); Inflammation analysis TNF-±, IL-1², IL-6, IL-10 and oxidative damage MDA will be evaluated.