Potential of different isolates of Metarhizium sp in the alternative control (induced resistance) of Phakopsora pachyrhizi in soybean plants

• Grant number: 18/18393-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2019
• End date: June 30, 2020
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Sérgio Florentino Pascholati
• Grantee: Sabrina Holz
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated scholarship(s): 19/19767-6 - Determine the active ingredient(s) of the anti-rust activity in the Metarhizium sp. culture filtrate and explore the potential of direct dsRNA application for managing soybean rust disease, BE.EP.MS

Abstract

The soybean rust, caused by Phakopsora pachyrhizi , Is one of the main diseases that affect the soybean crop, causing several losses. Thus, the search of new efficient management tools is increasing, notably the alternative methods as"Induced resistance and biological control", which consist in the use of agents to control a target pathogen. The entomopathogenic fungus Metarhizium sp. is used in the biological control of pests and exhibits the potential to be used against plant pathogens. Thus, the objective of this work is to evaluate the effect of preparations (media filtrate and conidial suspension) of three Metarhizium isolates (M. anisopliae, M. robertsii and Metarhizium indeterminated species 1) on the control of P. pachyrhizi, evaluating their potential use as inducer of systemic resistance, which activates the mechanisms of plant defense. For this purpose, susceptible plants (maintained under greenhouse conditions) and detached leaves (kept under laboratory conditions) will be used. Further studies regarding biochemical assays will be carried out to understand the basis of the phenomena. In vitro assays will be carried out to evaluate the inhibitory effect of the preparations on urediniospore germination. To evaluate the in vivo assays, the severity of the disease will be measure by using a diagrammatic scale. Based upon the results to be obtained, this project aim to contribute to the knowledge and development of an alternative control measure for the soybean rust. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HOLZ, SABRINA; D'ALESSANDRO, CELESTE PAOLA; MAXIMO, HEROS JOSE; DE SOUZA, PAULO HENRIQUE NASCIMENTO; RARUANG, YENJIT; DEMETRIO, CLARICE GARCIA BORGES; DELALIBERA JUNIOR, ITALO; CHEN, ZHI-YUAN; PASCHOLATI, SERGIO FLORENTINO. The potential of using Metarhizium anisopliae and Metarhizium humberi to control the Asian soybean rust caused by Phakopsora pachyrhizi. BIOCONTROL SCIENCE AND TECHNOLOGY, v. 33, n. 4, p. 17-pg., 2023-03-21. (18/18393-2, 19/23030-9, 19/19767-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

HOLZ, Sabrina. Potential of Metarhizium spp. isolates in the alternative control against Asian soybean rust. 2020. Master's Dissertation - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.