In vitro calogenesis as alternative to phyllanthine and hypophyllanthine production in Phyllantus amarus

• Grant number: 19/00243-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2019
• End date: March 31, 2020
• Field of knowledge: Agronomical Sciences - Agronomy
• Principal Investigator: Jean Carlos Cardoso
• Grantee: Maria Eduarda Barboza Souza de Oliveira
• Host Institution: Centro de Ciências Agrárias (CCA). Universidade Federal de São Carlos (UFSCAR). Araras , SP, Brazil

Abstract

The production of secondary metabolites of medicinal plants has increased its importance in the last decade. At the same time as the knowledge about its pharmacological properties gains space and market, the agriculture systems become the main source of these complex substances aiming its use for different purposes. In this context, the use of biotechnological techniques such as in vitro cultivation, associated with the induction of the production of secondary metabolites, requires knowledge on aspects related to plant physiology and that result in efficient methods of plant development control, associated to the induction of the production of secondary metabolites induced by chemical or physical agents, also known as elicitors. Thus, the present project aims at the in vitro production of secondary metabolites phyllanthin and hypophyllanthin obtained from Phyllanthus amarus species, using as tissues callus obtained from nodal segments. In order to do so, we are proposing experiments that involve: the establishment of a protocol aiming at obtaining callus for the production of vegetable mass, and then the tests with elicitors, aiming to evaluate the induction of the production of these metabolites in the callus. The system will be compared to a system of cultivation of potted plants under greenhouse conditions. In this way, it will be possible to evaluate the differences and viability of in vitro metabolite production compared to a conventional production system.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, BEATRIZ CRISTINA; BARBOZA SOUZA DE OLIVEIRA, MARIA EDUARDA; CARDOSO, JEAN CARLOS. Feasibility of the new method for orchid in vitro rooting using liquid and chemical sterilized culture medium under different sucrose concentration. ORNAMENTAL HORTICULTURE-REVISTA BRASILEIRA DE HORTICULTURA ORNAMENTAL, v. 25, n. 3, p. 263-269, JUL-SEP 2019. (19/00243-7)

CONDE DA SILVA DE MATOS, ANA VICTORIA; DE OLIVEIRA, BARBARA SAMANTHA; BARBOZA SOUZA DE OLIVEIRA, MARIA EDUARDA; CARDOSO, JEAN CARLOS. AgNO3 improved micropropagation and stimulate in vitro flowering of rose (Rosa x hybrida) cv. Sena. ORNAMENTAL HORTICULTURE-REVISTA BRASILEIRA DE HORTICULTURA ORNAMENTAL, v. 27, n. 1, p. 33-40, JAN-MAR 2021. (19/00243-7, 18/02595-5, 18/07122-8)

DE OLIVEIRA, MARIA EDUARDA B. S.; SARTORATTO, ADILSON; CARDOSO, JEAN CARLOS. In Vitro Calli Production Resulted in Different Profiles of Plant-Derived Medicinal Compounds in Phyllanthus amarus. Molecules, v. 25, n. 24, DEC 2020. (19/00243-7)

CARDOSO, JEAN CARLOS; DE OLIVEIRA, MARIA EDUARDA B. S.; CARDOSO, I, FERNANDA DE C.. Avanços e desafios na produção in vitro de metabólitos secundários de plantas medicinais. Horticultura Brasileira, v. 37, n. 2, p. 124-132, APR-JUN 2019. (19/00243-7)