Expression of P450 family genes in Brevipalpus yothersi (Acari: Tenuipalpidae) in response to different host plants

• Grant number: 19/03267-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2019
• End date: March 31, 2020
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Valdenice Moreira Novelli
• Grantee: Jéssica Gireli Bassani
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil

Abstract

The mites of the genus Brevipalpus are economically important due to polyphagia related to the vector capacity of phytoviruses known as VTBs (viruses transmitted by Brevipalpus). As main crops affected citrus, coffee, passion fruit and ornamental as the orchids. In citrus, leprosis, caused by citrus leprosis virus C (CiLV-C), is one of the most important viral diseases of orchards. From an atypical virus-vector relation, this disease is not systemic, that is, the virus does not circulate through the conducting vessels of citrus plants. The symptoms appear in the feeding places of the mite, forming chlorotic and / or necrotic lesions in the fruits, branches and leaves, culminating in stages advanced with the death of the plants. The management of this disease is primarily with the use of acaricides, being B. yothersi the species prevalent in the transmission of CiLV-C in commercial orchards in the state of São Paulo. However, little is known about the mechanisms of detoxification in these mites to xenobiotic compounds. At the Sylvio Moreira Citrus Center (CCSM-IAC), transcriptome (RNAseq) results identified differentially expressed genes (GDE) in B. yothersi, in response to CiLV-C. Among these genes, some of the cytochrome P450 family, with high similarity to the CYP3 clone of T. urticae, suggested a relationship with plant adaptation processes. Considering the importance of the leprosis mite also as vector of other VTBs, their high polyphagia and resistance potential in the orchards, the objectives of this proposal will be to evaluate the previously identified CYP3 family DDEs as regards the expression response in B. yothersi of different plants host, analyzing the involvement of these genes in detoxification processes. The results will contribute with unpublished information about this vector, expanding the perspectives of understanding the virus-vector-host interactions.