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Metabolomic and proteomic analysis in soccer players: relationships with aerobic and anaerobic capacities

Grant number: 19/02286-5
Support Opportunities:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): March 01, 2019
Effective date (End): July 31, 2022
Field of knowledge:Health Sciences - Physical Education
Acordo de Cooperação: Netherlands Organisation for Scientific Research (NWO)
Principal Investigator:Claudio Alexandre Gobatto
Grantee:João Pedro da Cruz
Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
Associated research grant:16/50250-1 - The secret of playing football: Brazil versus the Netherlands, AP.TEM


This project aims to identify and quantify the proteins and metabolites in the blood plasma of soccer players, through proteomic and metabolomic (Untargeted) techniques, and their relationship with the aerobic and anaerobic capacities obtained by the Critical Velocity protocol in field. For this, soccer players will be submitted to five evaluation sessions in field conditions. In the first session, they will receive information about the project, answer the anamnesis questionnaires, sports-competitive history and level of physical activity. In add, they will be submitted to body composition assessments. Blood samples will be collected for proteomic and metabolic analysis (Untargeted method). In the second, third, fourth and fifth sessions athletes will be submitted to predictive loads structured in a back-and-forth running protocol (20 meters), with four pre-established distances (800, 1200, 1600 and 2000 meters). In each of these predictive loads, players will be encouraged to run at maximum intensity for execution time to be recorded. A linear fit constructed using distance and time records will be applied to determine the angular coefficient (Critical Velocity or VCrit, equivalent to aerobic capacity) and linear coefficient (Anaerobic Running Capacity - ARC). In order to characterize the perceived exerction, Borg scale will also be applied before, during and after the exercises. With the adoption of computational analysis will be quantified and identified the metabolites and proteins in blood plasma, as well as their relationships with Vcrit and CCA. Our study aims to advance the theoretical and practical knowledge about soccer player characterization using biological Untargeted models and sport capacities. Such findings may, in the future, help the prescription and effectiveness of training reflecting on the performance of soccer players. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA CRUZ, JOAO PEDRO; DALCHECO MESSIAS, LEONARDO HENRIQUE; CETEIN, RAFAEL LUCAS; RASTEIRO, FELIPE MARRONI; GOBATTO, CLAUDIO ALEXANDRE; MANCHADO-GOBATTO, FULVIA BARROS. Anaerobic and Agility Parameters of Salonists in Laboratory and Field Tests. INTERNATIONAL JOURNAL OF SPORTS MEDICINE, v. 41, n. 7, p. 450-460, . (13/16710-7, 09/08535-5, 16/50250-1, 18/05821-6, 19/02286-5)
DA CRUZ, JOAO PEDRO; DOS SANTOS, FABIO NEVES; RASTEIRO, FELIPE MARRONI; MAROSTEGAN, ANITA BRUM; MANCHADO-GOBATTO, FULVIA BARROS; GOBATTO, CLAUDIO ALEXANDRE. A Metabolomic Approach and Traditional Physical Assessments to Compare U22 Soccer Players According to Their Competitive Level. BIOLOGY-BASEL, v. 11, n. 8, p. 16-pg., . (19/02286-5, 19/20894-2, 16/50250-1, 19/10666-2)

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