Potential of antimicrobial photodynamic therapy in the development of resistance in Candida albicans

• Grant number: 18/14874-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2019
• End date: February 29, 2020
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Ana Cláudia Pavarina
• Grantee: Luana Mendonça Dias Santana
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated research grant: 13/07276-1 - CEPOF - Optics and Photonic Research Center, AP.CEPID

Abstract

The study has the objective to evaluate the potential of an antimicrobial Photochemotherapy has to develop resistance of Candida albicans. Strains of resistant C. albicans (ATCC 96901) and susceptible (ATCC 90028) to fluconazole will be grown and exposed the antimicrobial Photochemotherapy (aPDT). After aPDT the colony forming units will be counted and survivors cells will be cultivated and exposed to the aPDT. This procedure will be repeated 10 times. The aPDT will be mediated by Photosensitizers (PSs) Curcumin and Photodithazine® associated to led light. Samples will be submitted to quantification of genetic expression by the technique of RT-qPCR, using specific primers for the genes related to the oxidative stress of the fungus, genes involved in the ergosterol synthesis, and the genes that express membrane transporter protein. Besides that, will also be evaluated the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (CFM), before and after the treatments to compare the fluconazole susceptibility between the strains (susceptible and resistant to fluconazole) of the groups evaluated. It will also be quantified the oxidative stress tolerance (ROS). The results will be evaluated by the most appropriate statistical method about the found data. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DIAS, LUANA MENDONCA; KLEIN, MARLISE INEZ; JORDAO, CLAUDIA CAROLINA; CARMELLO, JULIANA CABRINI; BELLINI, AMANDA; PAVARINA, ANA CLAUDIA. Successive applications of Antimicrobial Photodynamic Therapy effects the susceptibility of Candida albicans grown in medium with or without fluconazole. Photodiagnosis and Photodynamic Therapy, v. 32, DEC 2020. (16/13868-7, 18/14874-6)

DIAS, LUANA MENDONCA; KLEIN, MARLISE INEZ; FERRISSE, TULIO MORANDIN; MEDEIROS, KARINE SOUSA; JORDAO, CLAUDIA CAROLINA; BELLINI, AMANDA; PAVARINA, ANA CLAUDIA. The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer. JOURNAL OF FUNGI, v. 9, n. 1, p. 22-pg., 2023-01-01. (18/14874-6, 19/27634-6, 21/01191-0, 13/07276-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTANA, Luana Mendonça Dias. Potential of a antimicrobial photodynamic therapy in the development of resistance in Candida albicans. 2020. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.