Development an optical biossensor by surface plasmonic resonance (SPR) technique for the determination of creatine kinase (CK-MB)

• Grant number: 19/02692-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2019
• End date: January 31, 2021
• Field of knowledge: Engineering - Materials and Metallurgical Engineering
• Principal Investigator: Marystela Ferreira
• Grantee: André Lopes Ferreira
• Host Institution: Centro de Ciências e Tecnologias para a Sustentabilidade (CCTS). Universidade Federal de São Carlos (UFSCAR). Sorocaba , SP, Brazil

Abstract

The present work aims to develop an optical sensor by the surface plasmon resonance technique (SPR), which will be functionalized with cysteamine (Cys) and creatine phosphatase (Pcrea) biomolecules anchored to gold nanoparticles (AuNPs), seeking an increase in magnitude analytical signal and high specificity for detection of creatine Kinase (CK-MB). All steps will be monitored by displacement of the SPR angle, including steps of surface functionalization, deposition of materials on the substrate, and binder/analyte interaction. Auxiliary techniques will be used to characterize the biosensor, such as fluorescence spectroscopy, UV-vis spectroscopy, dynamic light scattering, microelectrophoresis, scanning electron microscopy (SEM) and electrochemical impedance. Based on the work proposal, the project can bring significant advances in the field of optical biosensors and in the detection of cardiac biomarker CK-MB, contributing to studies related to SPR spectroscopy in Brazil.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LIMA, LUCAS F. DE; DE FREITAS, AMANDA DE S. M.; MACIEL, CRISTIANE CARLA; FERREIRA, ANDRE L.; FERREIRA, MARYSTELA; ARAUJO, WILLIAM R. DE. Enzymeless glucose sensor based on disposable Ecoflex?/graphite thermoplastic composite substrate modified with Au@GQDs. SENSORS AND ACTUATORS REPORTS, v. 4, p. 11-pg., 2022-11-01. (18/08782-1, 19/02692-3, 18/04061-8)

DE LIMA, LUCAS F.; FERREIRA, ANDRE L.; MACIEL, CRISTIANE C.; FERREIRA, MARYSTELA; DE ARAUJO, WILLIAM R.. Disposable and low-cost electrochemical sensor based on the colorless nail polish and graphite composite material for tartrazine detection. Talanta, v. 227, MAY 15 2021. (19/02692-3, 18/08782-1, 18/04061-8)

MACIEL, CRISTIANE C. C.; FERREIRA, ANDRE L. L.; FERREIRA, MARYSTELA; DE LIMA, LUCAS F.; DE ARAUJO, WILLIAM R.. Development of a flexible and disposable electrochemical sensor based on poly (butylene adipate-co-terephthalate) and graphite for hydroquinone sensing. SENSORS AND ACTUATORS REPORTS, v. 4, p. 10-pg., 2022-11-01. (19/02692-3, 18/04061-8)

FERREIRA, ANDRE L.; DE LIMA, LUCAS F.; MORAES, ARIANA S.; RUBIRA, RAFAEL J. G.; CONSTANTINO, CARLOS J. L.; LEITE, FABIO L.; DELGADO-SILVA, ADRIANA O.; FERREIRA, MARYSTELA. Development of a novel biosensor for Creatine Kinase (CK-MB) using Surface Plasmon Resonance (SPR). Applied Surface Science, v. 554, p. 9-pg., 2021-03-26. (16/19387-0, 18/04061-8, 19/02692-3, 14/50869-6)

DE LIMA, LUCAS FELIPE; FERREIRA, ANDRE LOPES; DE FREITAS, AMANDA DE SOUSA MARTINEZ; RODRIGUES, JESSICA DE SOUZA; LEMES, ANA PAULA; FERREIRA, MARYSTELA; DE ARAUJO, WILLIAM REIS. Biodegradable and Flexible Thermoplastic Composite Graphite Electrodes: A Promising Platform for Inexpensive and Sensitive Electrochemical Detection of Creatine Kinase at the Point-of-Care. ACS APPLIED MATERIALS & INTERFACES, v. 15, n. 15, p. 13-pg., 2023-04-04. (20/12659-0, 16/19387-0, 19/02692-3, 22/03250-7, 18/08782-1, 18/04061-8)