Understanding the biophysiochemical interactions between plasmonic nanoparticles and enzymes to obtain bio-nanomaterials with controlled properties

• Grant number: 19/09668-0
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: September 01, 2019
• End date: August 31, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Susana Inés Córdoba de Torresi
• Grantee: Heloise Ribeiro de Barros
• Supervisor: Luis Manuel Liz Marzan
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Centre for Cooperative Research in Biomaterials (CIC biomaGUNE), Spain
• Associated to the scholarship: 17/20892-4 - Nanobioplasmonic catalysts: a novel approach to controlling the selectivity in light-driven one-pot reactions, BP.PD

Abstract

Controlling biophysiochemical interactions between plasmonic nanoparticles and biomolecules is a promising alternative tool to design bio-nanomaterials with desirable properties. Moreover, the understanding of these interactions is a key factor to the development of nanoengineered materials for several applications, especially in the biological field. However, the effective control of these properties is still challenging in colloidal material science. Therefore, the objective of this project is to build up robust nanomaterials composed by plasmonic nanoparticles and enzymes controlled by their biophysiochemical interactions upon external stimuli. As a proof-of-concept, gold nanoparticles (AuNPs) functionalized with glucose-oxidase (GOD) and lipase from Candida antarctica fraction B (CALB) will be used as model systems to understand the effects promoted in their properties upon external stimuli. Light stimulus will be used to study the enzymatic performance of GOD-AuNPs due to the excitation of the local surface plasmon resonance (LSPR) of AuNPs. Temperature stimulus will be explored to trigger interaction changes in CALB-AuNPs in order to obtain materials with plasmon-enhanced chiroptical activities. Then, we expected to obtain the precise control over the biophysiochemical interactions between AuNPs and GOD and CALB upon the use of light and temperature. Furthermore, the control of these properties plays a crucial role in the design and application of novel bio-nanomaterials. Finally, this study represents an opened opportunity to the development of more elaborated materials involving enzymes and AuNPs with tailored properties by controlling their biophysiochemical properties.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, RAFAEL T. P.; DE BARROS, HELOISE. R. R.; FERNANDES, RAFAELLA. F. F.; TEMPERINI, MARCIA L. A.; DE TORRESI, SUSANA. I. CORDOBA I.. Cytochrome C with Peroxidase-like Activity Supported on Plasmonic AuNPs: Improved Stability and Enhanced Nanobioplasmonic Catalytic Conversion. CHEMCATCHEM, v. N/A, p. 9-pg., 2023-02-01. (18/25422-9, 19/09668-0, 18/13492-2, 16/21070-5, 17/20892-4, 15/26308-7)

BARROS, HELOISE RIBEIRO; TANAKA, LIVIA YUKARI; PACHECO DA SILVA, RAFAEL TRIVELLA; SANTIAGO-ARCOS, JAVIER; CORDOBA TORRESI, I, SUSANA; LOPEZ-GALLEGO, FERNANDO. Assembly of Nano-Biocatalyst for the Tandem Hydrolysis and Reduction of p-Nitrophenol Esters. PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION, v. 38, n. 10, AUG 2021. (19/19551-3, 18/02435-8, 17/20892-4, 15/26308-7, 18/13492-2, 19/09668-0)

RIBEIRO DE BARROS, HELOISE; GARCIA, ISABEL; KUTTNER, CHRISTIAN; ZEBALLOS, NICOLL; CAMARGO, PEDRO H. C.; CORDOBA DE TORRESI, SUSANA INES; LOPEZ-GALLEGO, FERNANDO; LIZ-MARZAN, LUIS M.. Mechanistic Insights into the Light-Driven Catalysis of an Immobilized Lipase on Plasmonic Nanomaterials. ACS CATALYSIS, v. 11, n. 1, p. 414-423, JAN 1 2021. (18/13492-2, 19/09668-0, 15/26308-7, 17/20892-4)

DE BARROS, HELOISE RIBEIRO; LOPEZ-GALLEGO, FERNANDO; LIZ-MARZAN, LUIS M.. Light-Driven Catalytic Regulation of Enzymes at the Interface with Plasmonic Nanomaterials. BIOCHEMISTRY, v. 60, n. 13, p. 991-998, APR 6 2021. (19/09668-0)