SERS performance of AuNP encapsulated in alginate and chitosan templates for drugs detection

• Grant number: 19/05989-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2019
• End date: November 30, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Marcia Laudelina Arruda Temperini
• Grantee: Mari Ferreira Nicolas
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 16/21070-5 - Vibrational spectroscopy with spatial resolution, AP.TEM

Abstract

The development of biological sensors for studying drug monitoring, disease detection and increased sensitivity to the dosage of these drugs are being extensively studied, however finding a substrate that is biocompatible and at the same time, efficient still remains a challenge. Polysaccharides such as alginate and chitosan are widely used for this purpose due to their low cytotoxicity, encapsulating and gelling power (ion exchange in the presence of salts such as CaCl2). When adsorbed on metallic nanoparticle (NPM) colloids such as Au promote the stabilization of NPM, a fundamental factor for the formation of substrates, but their effects for SERS technique application are still little known. The understanding of the effect of the composition of these polysaccharides on the SERS substrates formed by Au and alginate and/or chitosan nanostructure on the quality and reproducibility of the spectra of the biomolecule of interest is the goal of this project. The aim of this study is to determine the most suitable system for the manufacture of biosensors by the SERS technique using the chitosan and alginate polysaccharides, separated or combined, in order to detect antibiotics in commercial samples.