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Effect of probiotics on the genus Lactobacillus on oral colonization and spread of P. gingivalis systemic in experimental periodontitis model in mice

Grant number: 19/05256-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2019
Effective date (End): February 28, 2021
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Marcia Pinto Alves Mayer
Grantee:Marina Paraluppi
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:15/18273-9 - New strategies for the control of periodontitis, AP.TEM

Abstract

The aim of this study is to test the hypothesis that distinct of P. gingivalis strains differ in capacity for colonisation of the oral cavity and distant sites and determine the effect of probiotic strains of the genus Lactobacillus on colonization systemic and oral of P. gingivalis capsulated and non-capsulated in experimental periodontitis model using two strains of P. gingivalis. The effect of oral administration of L. acidophilusLA5 and L. rhamnosusLR32 on P. gingivalis levels will be determined in mice C57Bl6 submitted to experimental periodontitis induced by oral infection visa [P. gingivalis ATCC 33277 (strain fimbriada, fimA genotype 1), and cepa W83 (capsulated, fimA genotype IV)]; Prevotella intermedia, F.nucleatum ssp. Nucleatum ATCC 25586 e S. gordoniiDL1). After 42 days, will be held the euthanasia, collected oral biofilm samples, blood, gingival tissue, liver, spleen, intestine, and brain and will be assessed the total levels of p. gingivalis (W83 + ATCC 33277) and cepa capsulated (W83, fimA genotype IV), by PCR quantitative.