Oxygen-ozone therapy for treatment of induced Ischemic Cerebrovascular Accident in an in vitro animal model

• Grant number: 18/24552-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2019
• End date: May 31, 2022
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Carlos Eduardo Ambrósio
• Grantee: Jéssica Rodrigues Orlandin
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

Encephalic Vascular Accident, or stroke, is the most common pathology of the central nervous system in humans, being the second leading cause of death and physical and cognitive disabilities in developing countries. It is a vascular disorder, which may present in an ischemic or hemorrhagic form, being the first one more common. Although there is still a lack of studies on the prevalence of strokes in animals, it is believed that the pathology is becoming increasingly common, mainly due to the habits and progressive age of dogs and cats. The present study aims to evaluate in vitro the effect of ozone therapy treatment after induced cerebral ischemia. Neuroblastoma lineage cells (SH-SY5Y) were cultured in 96 microwell plates and subjected to 24 hours of hypoxia in an incubator culture chamber. After this period, the cells were treated for 24 hours with different concentrations of ozone. Through cell metabolic analysis, performed by colorimetric assay, it was possible to observe that at higher concentrations, ozone has a harmful effect on these cells, while at lower concentrations, they provided cell growth, indicating a possible neuro-regenerative effect of ozone. A pilot study was carried out in vivo, in 5 rats, which were submitted to cerebral ischemia. This study will not continue due to the high mortality rate, not allowing us to obtain many results. We propose to carry out more in vitro analyzes in order to obtain other parameters that allow us to define an ideal therapeutic range for the treatment of cerebral ischemia. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ORLANDIN, JESSICA RODRIGUES; PINTO SANTOS, SARAH INGRID; MACHADO, LUCIANA CRISTINA; NETO, PAULO FANTINATO; BRESSAN, FABIANA FERNANDES; GODOY PIERI, NAIRA CAROLINE; RECCHIA, KAIANA; COUTINHO, MELINE DE PAULA; FERREIRA PINTO, PRISCILLA AVELINO; SANTUCCI, ANNALISA; et al. Evaluation of targeted oxidative stress induced by oxygen-ozone in vitro after ischemic induction. Redox Report, v. 27, n. 1, p. 11-pg., 2022-12-31. (18/24552-6)

ORLANDIN, JESSICA RODRIGUES; MACHADO, LUCIANA CRISTINA; AMBROSIO, CARLOS EDUARDO; TRAVAGLI, VALTER. Ozone and its derivatives in veterinary medicine: A careful appraisal. VETERINARY AND ANIMAL SCIENCE, v. 13, SEP 2021. (18/24552-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ORLANDIN, Jéssica Rodrigues. Oxygen-Ozone therapy for treatment of induced Ischemic Stroke in an in vitro animal model. 2022. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Zootecnica e Engenharia de Alimentos (FZE/BT) Pirassununga.