| Grant number: | 19/08239-9 |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| Start date: | June 01, 2019 |
| End date: | June 30, 2024 |
| Field of knowledge: | Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms |
| Principal Investigator: | Marie-Anne Van Sluys |
| Grantee: | Henrique Moura Dias |
| Host Institution: | Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Associated research grant: | 16/17545-8 - Genes, genomes & transposable elements contribution to plant-microbe interaction: a sugarcane study case, AP.BIOEN.TEM |
| Associated scholarship(s): | 22/16208-9 - Pan grass syntenic gene set based on sugarcane inclusion and the thiamine biosynthesis pathway, BE.EP.DD |
Abstract The THI1 gene encodes a thiazole biosynthesis protein, a component of thiamine (vitamin B1). Thiamine is an essential cofactor in metabolic carbohydrates and amino acids, especially in plants. In A. thaliana there is only one copy of the THI1 gene and its primary transcript is directed to the chloroplasts and mitochondria through the use of two initialization codons. In addition, the A. thaliana tz-201 (thiamine autotrophic) mutant accumulates more sucrose in its tissues than a wild plant. The moss Physcomitrela patens although their reduced complexity compared to higher plants, 6 genomic copies of the THI1 gene were identified in their genome through BLAST, where it is believed to be an important clue as to the evolution of this gene in plants. In sugarcane, 3 copies of THI1 were identified in BAC analysis (Bacterial Artificial Chromosome), which are responsive to biotic stress caused by Sporisorium scitamineum infection late. This suggests that a better understanding of the THI1 gene and its role in the accumulation of sucrose and biotic stress may be important for the commercial improvement of cultivars. Due to the identification of the THI1 gene in A. thaliana, possibly associated with DNA and carbon pathway repair in plants, it is necessary to study the function of this gene. For this, individual knockout lines of Physcomitrella patens were obtained for the six copies of the THI1 gene, where they will be characterized in this work for later study of functional complementation in experiments that allow the understanding of the specific functions of different homologues of the THI1 gene family in plants, in especially sugarcane. (AU) | |
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