A fully differentiated cell does not, naturally, return to cellular pluripotency. Although, somatic nuclear transfer, cloning, and other methodologies based on exogenous expression, shows cellular reversibilitty through nuclear reprogramming. Embryonic development and cell differentiation originate complex tissues and structures from gene expression. Furthermore, these processes are regulated by a series of complex genetic and epigenetic mechanisms, including, among others, the long non-coding RNAs (lncRNA). LncRNAs are a relatively new class of noncoding RNAs with increasing scientific interest, due to its embryonic development and gene regulation role. However, little is known about its role in bovine embryonic development. For the present project, we hypothesized that nuclear reprogramming and early bovine embryonic development are also regulated by lncRNA present in gametes and inherited to the zygote. Using transcriptomics, bioinformatics and reverse engineering techniques, we aim to identify and predict lncRNA expressed in gametes and zygotes. Hoping to determine these lncRNAs importance during early embryonic development and bovine nuclear reprogramming.
News published in Agência FAPESP Newsletter about the scholarship: