Analysis of differentially expressed genes in PRNPlow and PRNPhigh patient-derived glioblastoma stem cells

• Grant number: 19/14552-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2019
• End date: July 31, 2020
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Marilene Hohmuth Lopes
• Grantee: Giulia La Rocca Mazzaro
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/15557-4 - Prion protein and its partners: emerging targets for glioblastoma stem cell based-therapy, AP.JP2

Abstract

The Glioblastoma Multiforme is a highly aggressive tumor, with a high recurrence and death rates. Studies have shown that the GBM is sustained by a subpopulation of cells with stem cells- like characteristics, denominated Glioblastoma Stem Cells. Those cells are resistant to chemo and radiotherapy, and are responsible for the low success rate of treatments in this tumor. Our group has described the role of the Protein Prion Celular (PrPC) in the biology of the GSC, being involved in the modulation of proliferation and self-renewal of those cells. The PrPC is a GPI- anchored protein, capable of acting as a scaffold protein, forming complexes with multiple proteins, such as stemness markers. Our group has described a heterogeneous gene (PRNP) and protein expression of PrPC in glioblastoma samples being its high expression correlated with increased tumor proliferation and decreased patient survival. Thus, the main goal of this project is to evaluate the differentially expressed genes in GSCs expressing distinct PrPC levels to identify novel potential targets of PrPC.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

COELHO, BARBARA PARANHOS; DE LIMA FERNANDES, CAMILA FELIX; BOCCACINO, JACQUELINE MARCIA; DA SILVA SOUZA, MARIA CLARA; MELO-ESCOBAR, MARIA ISABEL; ALVES, RODRIGO NUNES; PRADO, MARIANA BRANDAO; IGLESIA, REBECA PIATNICZKA; CANGIANO, GIOVANNI; MAZZARO, GIULIA LA ROCCA; et al. Multifaceted WNT Signaling at the Crossroads Between Epithelial-Mesenchymal Transition and Autophagy in Glioblastoma. FRONTIERS IN ONCOLOGY, v. 10, NOV 12 2020. (19/14552-1, 19/14952-0, 17/26158-0, 20/04687-4, 19/06971-4, 19/11097-1, 19/12710-9, 20/07450-5, 18/15557-4, 19/14741-9)