Influence of fluoride- and stannous-containing products on the acquired salivary pellicle thickness and loosely bound fluoride on enamel - an in situ study

• Grant number: 19/11368-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): October 01, 2019
• Effective date (End): March 31, 2020
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Taís Scaramucci Forlin
• Grantee: Alana Cristina Machado
• Supervisor: Bennett Tochukwu Amaechi
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Research place: University of Texas Health Science Center at San Antonio (UTHSCSA), United States
• Associated to the scholarship: 17/24714-3 - Effect of the order of application of dentifrices and solutions containing fluorides and tin in the progression of erosive wear on enamel and bovine dentin - in situ study, BP.DR

Abstract

This study aims to evaluate the AEP thickness after treatment with a fluoride plus stannous dentifrice and mouthrinse, and the amounts of KOH-soluble fluoride on enamel after treatment with these products in the presence and absence of the AEP. For this, a 3-phase, single bind, cross over in situ trial, involving 12 volunteers. The experimental groups will be the following: B+R- toothbrushing with F+Sn dentifrice, followed by rinse with F+Sn solution; R+B- rinse with F+Sn solution, followed by toothbrushing with F+Sn dentifrice and C: Control (toothbrushing with artificial saliva). The voluntaries will use two mandibular devices containing 6 specimens each one. The devices containing the specimens will be firstly immersed in 20ml of the 0.3% citric acid (pH=2.6) solution extra-orally, for 5min. One device will be placed into the mouth and remain there for 1h, to allow the formation of the AEP. The other device will be kept under humid environment (without pellicle). After, two specimens will be removed from each of the devices. The remaining specimens from both devices will be then exposed intra-orally to 10ml of the mouthrinse for 30s or will be brushed for 5s with the dentifrice and the suspension will kept in the mouth until a total of 2 min, depending on the study phase. After, two more specimens will be removed from each device. In the sequence, the volunteer that performed the rinse will brush and vice-versa. The final two specimens from each side will then be removed. The control group will only hold 4 specimens at each side and will be brush with saliva only and there would be no rinse. Pellicle thickness will be measured by confocal laser scanning microscopy (CLSM) and the fluoride content in the solution will be measured with a fluoride selective electrode. If normality and homoscedasticity assumptions were satisfied, data will be analyzed by two ANOVA repeated measures and Tukey tests. The level of significance will be 5%.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MACHADO, ALANA CRISTINA; PHILLIPS, THAIS SANTIAGO; ZIMMERMAN, RICHARD; SCARAMUCCI, TAIS; AMAECHI, BENNETT TOCHUKWU. Monitoring erosive tooth wear with intraoral 3D scanner: A feasibility study. AMERICAN JOURNAL OF DENTISTRY, v. 35, n. 1, p. 6-pg., 2022-02-01. (17/24714-3, 19/11368-5)