Reconstruction of the gene regulatory network in Pseudomonas aeruginosa

• Grant number: 19/14883-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2019
• End date: February 29, 2020
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Rafael Silva Rocha
• Grantee: Bruno Rossi Carmo
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 12/22921-8 - Synthetic biology approaches for deciphering the logic of signal integration in complex bacterial promoters, AP.JP

Abstract

Pseudomonas aeruginosa is a bacterium often found in the environment and opportunistic pathogen associated with serious infections. This organism has a genome of more than 6 Mb which is controlled by sophisticated molecular machinery that includes ~25 sigma factors and hundreds of transcription factors. Although many studies have investigated several transcription factors related to the lifestyle of this organism, few studies aim at the global reconstruction of the regulatory network of this bacterium. Thus, the present project aims to investigate the global P. aeruginosa network by integrating available high-throughput data from specialized databases. This approach is expected to increase the understanding of the molecular mechanisms related to the control of gene expression in this bacterium.