Morphological and molecular characteristics of soil mesostigmatic mites taken from different agricultural environments and in the natural vegetation of the Atlantic forest biome in the state of Sao Paulo

• Grant number: 19/12175-6
• Support type: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): October 10, 2019
• Effective date (End): March 21, 2020
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal researcher: Raphael de Campos Castilho
• Grantee: Emiliano Brandão de Azevedo
• Supervisor abroad: Marie-Stephane Tixier Lopes
• Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Research place: Centre de Biologie et de Gestion des Populations (CBGP), France
• Associated to the scholarship: 18/14478-3 - Effect of agricultural crops on the diversity of Mesostigmata predator mites and prospecting for use in biological control, BP.DR

Abstract

The edaphic acarofauna is little known in the world, even in Brazil. Mesostigma are the second most abundant group of mites in the soil and may play an important role in the regulation of pests as predators. The objective of this work is to characterize the species present in different agricultural environments and in an area of natural vegetation of the Atlantic Forest biome using morphological and molecular approaches. The collection will be carried out in four areas of corn and soybean, as well as in an area of natural vegetation. In each area, soil samples will be collected monthly from December 2017 to October 2019 and the mites will be extracted using a modified Berlese funnel (containing an absolute alcohol vial). The samples will be examined and the mesostigmas will be isolated in tubes containing absolute alcohol. Part of the mites will be mounted on slides and coverslips for morphological identification. The other part will be used for molecular analysis at the Center de Biologie pour l'Gestion des Populations (CBGP) / Montpellier SupAgro in Montpellier, France. The DNA of each mite will be individually extracted and several markers (mitochondrial and nuclear) will be used for molecular characterization (sequencing). Each new species identified will be described. The results will be published in scientific articles in international or national journals with high impact factor and will be presented at congresses.