Role of Sirtuin 1/Sirtuin 3 in response to obesity and aging-associated inflammation

• Grant number: 19/15411-2
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: October 01, 2019
• End date: September 30, 2020
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Niels Olsen Saraiva Câmara
• Grantee: Omar Alberto Domínguez Amorocho
• Supervisor: Maria Mittelbrunn Herrero
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Universidad Autónoma de Madrid (UAM), Spain
• Associated to the scholarship: 17/16711-4 - Role of sirtuin 1 in CD4+ T cell activation and differentiation in obese transplanted engrafted animals, BP.DR

Abstract

Histone deacetylases (HDACs) are biological molecules responsible for removing acetyl group from lysine residues of histone. Sirtuin1 (Sirt1) and Sirtuin3 (Sirt3) are two well-characterized members of the silent information regulator 2 (Sir2) family of proteins, involved in vital roles in resistance to cellular stress, but the role of these sirtuins upon inflammatory stress is not fully understand. Then, the present study pretends to investigate the SIRT1SIRT3 axis employed by the cell to respond to stressful stimuli derived from obesity and aging. Effect of SIRT1 and SIRT3 alterations on CD4+ T cells as a cause of obesity and aging will be determined by the techniques of flow cytometry, RT-PCR and cell bioenergetics assays to measure CD4+ T cell phenotypes based in cytokine production, expression of transcription factors and metabolic profile. We expected to unravel the mechanisms behind the role of SIRT1/Sirt3 axis in CD4 mediated responses and add new innovative data on this field.