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Effect of low-intensity laser irradiation on the quality of the induced membrane in Masquelet's technique

Grant number: 19/01750-0
Support type:Scholarships in Brazil - Master
Effective date (Start): September 01, 2019
Effective date (End): February 28, 2021
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal Investigator:Sheila Canevese Rahal
Grantee:Jeniffer Gabriela Figueroa Coris
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


This study aimed to evaluate the influence of low-intensity laser irradiation on the quality of the Masquelet membrane induced in orthotopic site in rabbits. Twelve Norfolk rabbits, females, at the age around 60 days and weighing 3-3.5 kg will be used. In both forelimbs, a segmental bone defect of 1.0 cm length will be induced in the radius that will be filled with a cylinder of polymethylmethacrylate bone cement. Low-level laser therapy will be used postoperatively in the bone defect of one of the forelimbs by point technique. The pen will be positioned perpendicular to the center of the segmental defect. Infrared radiation will be used for 20 seconds, providing 2J of energy, every 48 hours, during 15 days. The areas of the bone defects will be evaluated by ultrasound and radiographic examinations performed periodically. Six rabbits will be submitted to euthanasia at three weeks postoperatively and six rabbits at six weeks postoperatively to perform the histological and immunohistochemical examinations of the Masquelet's membranes. The presence of inflammatory reaction, foreign body reaction and fibrosis, as well as the vascular endothelial growth factors and platelet-derived growth will be assessed. The obtained data will be evaluated by statistical analysis. (AU)