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| Author(s): |
Jeniffer Gabriela Figueroa Coris
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Botucatu. 2021-01-13. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu |
| Defense date: | 2020-12-02 |
| Advisor: | Sheila Canevese Rahal |
| Abstract | |
This study aimed to evaluate the influence of low-intensity laser irradiation on the development and characteristics of the Masquelet membrane induced in orthotopic site in rabbits. Twelve Norfolk rabbits, mean age of 3 months were used. In both forelimbs, a segmental bone defect of 1.0 cm length was induced in the radius, which was filled with a cylinder of polymethylmethacrylate bone cement. Low-level laser therapy was used postoperatively in the bone defect of one of the forelimbs by point technique (2J energy) every 48 hours for 15 days. The bone defect were evaluated by ultrasound and radiographic examinations. Six rabbits were submitted to euthanasia at three weeks postoperatively and six rabbits at six weeks postoperatively to perform the histological and immunohistochemical examinations of the Masquelet’s membranes. In both forelimbs, radiographs showed new bone growth from the cut ends of the radius on third postoperative week, and more advanced stage on sixth postoperative week. Sonographic examination identified in both forelimbs the presence of the induced membrane one week after the surgery. Histologically, there were no statistically significant differences in semi-quantitative score of inflammation intensity, total number of blood vessels, bone metaplasia and collagen. The average thickness of the induced membranes was 2050.17 µm and 1451.96 µm in the control forelimb and 2724.26 µm and 2081.03 µm in the laser irradiated limb, respectively on third and sixth postoperative weeks. VEGF and PDGF expression were present in the induced membranes of the control and irradiated forelimbs, but showed no significant difference. In conclusion, based on assessment methods was not possible to demonstrate statistically the influence of low-intensity laser irradiation in the Masquelet membrane. (AU) | |
| FAPESP's process: | 19/01750-0 - Effect of low-intensity laser irradiation on the quality of the induced membrane in Masquelet's technique in rabbits |
| Grantee: | Jeniffer Gabriela Figueroa Coris |
| Support Opportunities: | Scholarships in Brazil - Master |