Fluorescence of Bacillus subtilis for endophytic analysis and growth promotion in corn plants

• Grant number: 19/14238-5
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): October 01, 2019
• Effective date (End): November 30, 2020
• Field of knowledge: Agronomical Sciences - Agronomy
• Principal Investigator: Everlon Cid Rigobelo
• Grantee: Clara Barros Bueno
• Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The use of microorganisms as promoters of plant growth in agriculture has been expanding in Brazil and several studies point to a good performance of these microorganisms on agricultural productivity in the field. These good results provide a real possibility of reducing the use of mineral fertilizers in agricultural production. These microorganisms are most often found in the rhizosphere where most plant-microorganism relationships occur. Studies show that depending on the ability of the microorganism, the plant and the interaction, some rhizospheric microorganisms enter the plant tissue and colonize it. From that moment on these rhizospheric microorganisms become endophytic. Some bacteria have the capacity of endofitismo, that is, occupy vegetative tissues without causing any damage to the host, on the contrary, it is known that plants use more efficiently the phytohormones synthesized by the endophytic bacteria and as a consequence, a greater development takes place radicular, overhead, more efficient use of mineral fertilizers and soil water. However, it is not known whether inoculant dose and soil fertility conditions interfere with endophytic disease. In view of the above, the present study aims to verify if the endophytic is influenced by the dose of the bacterial inoculant and by the soil fertility conditions and if the plant development is influenced by the endophytic bacterial population. For this, the plant development of soybean and corn plants in concentrations of Bacillus subtilis labeled with GFP protein at doses of 1 x 102 to 1 x 1010 UFC.mL-1 in four levels of mineral fertilization will be evaluated. , 25%, 50% and 100% of the fertilizer dose according to the chemical analysis of the soil under conditions of each vegetation.