Study of the Mechanisms of Action in Photodynamic Therapy: From Photosensitizer to Practical Application

• Grant number: 19/13569-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: September 01, 2019
• End date: March 31, 2022
• Field of knowledge: Physical Sciences and Mathematics - Physics
• Principal Investigator: Vanderlei Salvador Bagnato
• Grantee: Lucas Danilo Dias
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 13/07276-1 - CEPOF - Optics and Photonic Research Center, AP.CEPID

Abstract

Photodynamic therapy (PDT) is an alternative, selective and non-toxic technique that is applied to destroy cancer cells as well as pathogenic microorganisms, by light activation of a photosensitizer molecule. Its operation requires the conjunction of three fundamentals components: ideal photosensitizer, appropriate wavelength light source and oxygen. This project intends to carry out a systematic in vitro study regarding the ptimization/selection of photosensitizer structure/light source, to infer about the relationship between the mechanism of action of photodynamic therapy and the cellular location of the photosensitizer.Functionalization and characterization methods (UV-Vis, Infra-red and Raman spectroscopy) of solid supports / biomedical devices with optimal photosensitizers for the development of materials with biomedical application will be evaluated. Among them, we highlight solid biomaterials bearing immobilized photosensitizers capable of possessing antimicrobial properties by photodynamic action. In vitro studies will be carried out in strains of opportunistic bacteria causing skin and upper respiratory tract infections, such as Staphylococcus aureus, Eccherichia coli, Pseudomonas aeruginosa and Streptococcus pyogenes, and in addition, all cytotoxicity assays in human fibroblast cells will be performed. The most promising material(s) will be applied for in vivo (animal models) evaluation.

Scientific publications

(28)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES, FABIO M. S.; DIAS, LUCAS D.; CALVETE, MARIO J. F.; MARIA, TERESA M. R.; ROSSI, LIANE M.; POMBEIRO, ARMANDO J. L.; MARTINS, LUISA M. D. R. S.; PEREIRA, MARIETTE M.. Immobilization of Rh(I)-N-Xantphos and Fe(II)-C-Scorpionate onto Magnetic Nanoparticles: Reusable Catalytic System for Sequential Hydroformylation/Acetalization. CATALYSTS, v. 11, n. 5, MAY 2021. (16/16738-7, 19/13569-8)

SEPULCHRO, V, ANA GABRIELA; PELLEGRINI, VANESSA O. A.; DIAS, LUCAS D.; KADOWAKI, MARCO A. S.; CANNELLA, DAVID; POLIKARPOV, IGOR. Combining pieces: a thorough analysis of light activation boosting power and co-substrate preferences for the catalytic efficiency of lytic polysaccharide monooxygenase MtLPMO9A. BIOFUEL RESEARCH JOURNAL-BRJ, v. 8, n. 3, p. 1454-1464, SUM 2021. (18/22300-0, 15/13684-0, 19/13569-8)

FIRMINO, POLLYANA P.; QUEIROZ, JAQUELINE E.; DIAS, LUCAS D.; WENCESLAU, PATRICIA R. S.; DE SOUZA, LARISSA M.; IERMAK, IEVGENIIA; VAZ, WESLEY F.; CUSTODIO, JEAN M. F.; OLIVER, ALLEN G.; DE AQUINO, GILBERTO L. B.; et al. Synthesis, Molecular Structure, Thermal and Spectroscopic Analysis of a Novel Bromochalcone Derivative with Larvicidal Activity. CRYSTALS, v. 12, n. 4, p. 18-pg., 2022-04-01. (13/07276-1, 14/50857-8, 19/13569-8)

SOARES, JENNIFER MACHADO; INADA, NATALIA MAYUMI; BAGNATO, VANDERLEI SALVADOR; BLANCO, KATE CRISTINA. Evolution of surviving Streptoccocus pyogenes from pharyngotonsillitis patients submit to multiple cycles of antimicrobial photodynamic therapy. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 210, SEP 2020. (19/13569-8, 13/07276-1)

DIAS, LUCAS D.; BAGNATO, VANDERLEI S.. An update on clinical photodynamic therapy for fighting respiratory tract infections: a promising tool against COVID-19 and its co-infections. LASER PHYSICS LETTERS, v. 17, n. 8, AUG 2020. (19/13569-8, 13/07276-1, 14/50857-8)

DIAS, LUCAS D.; BLANCO, KATE C.; MFOUO-TYNGA, IVAN S.; INADA, NATALIA M.; BAGNATO, VANDERLEI S.. Curcumin as a photosensitizer: From molecular structure to recent advances in antimicrobial photodynamic therapy. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY C-PHOTOCHEMISTRY REVIEWS, v. 45, DEC 2020. (19/14885-0, 13/07276-1, 14/50857-8, 19/12694-3, 19/13569-8)

DIAS, LUCAS D.; MFOUO-TYNGA, IVAN S.. Learning from Nature: Bioinspired Chlorin-Based Photosensitizers Immobilized on Carbon Materials for Combined Photodynamic and Photothermal Therapy. BIOMIMETICS, v. 5, n. 4, DEC 2020. (19/13569-8, 14/50857-8, 19/14885-0, 13/07276-1)

DIAS, LUCAS D.; RODRIGUES, FABIO M. S.; CALVETE, MARIO J. F.; CARABINEIRO, SONIA A. C.; SCHERER, MARISA D.; CAIRES, ANDERSON R. L.; BUIJNSTERS, JOSEPHUS G.; FIGUEIREDO, JOSE L.; BAGNATO, VANDERLEI S.; PEREIRA, MARIETTE M.. Porphyrin-Nanodiamond Hybrid Materials-Active, Stable and Reusable Cyclohexene Oxidation Catalysts. CATALYSTS, v. 10, n. 12, DEC 2020. (13/07276-1, 14/50857-8, 19/13569-8)

WILLIS, JACE A.; CHEBURKANOV, VSEVOLOD; KASSAB, GIULIA; SOARES, JENNIFER M.; BLANCO, KATE C.; BAGNATO, VANDERLEI S.; YAKOVLEV, VLADISLAV V.. Photodynamic viral inactivation: Recent advances and potential applications. APPLIED PHYSICS REVIEWS, v. 8, n. 2, JUN 2021. (18/18188-0, 13/07276-1, 19/13569-8)

DIAS, LUCAS D.; CORREA, THAILA Q.; BAGNATO, VANDERLEI S.. Cooperative and competitive antimicrobial photodynamic effects induced by a combination of methylene blue and curcumin. LASER PHYSICS LETTERS, v. 18, n. 7, JUL 2021. (14/50857-8, 13/07276-1, 19/13569-8)

QUEIROZ, JAQUELINE E.; DIAS, LUCAS D.; VILA VERDE, GIULIANA M.; AQUINO, GILBERTO L. B.; CAMARGO, ADEMIR J.. An Update on the Synthesis and Pharmacological Properties of Pyrazoles Obtained. CURRENT ORGANIC CHEMISTRY, v. 26, n. 2, p. 10-pg., 2022-01-01. (19/13569-8)

DA SILVA, ANA PAULA; ULIANA, MARCIANA PIERINA; GONTIJO GUIMARAES, FRANCISCO EDUARDO; DE OLIVEIRA, KLEBER THIAGO; BLANCO, KATE CRISTINA; BAGNATO, VANDERLEI SALVADOR; INADA, NATALIA MAYUMI. Investigation on the in vitro anti-Trichophyton activity of photosensitizers. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, v. 21, n. 7, p. 8-pg., 2022-03-24. (13/07276-1, 11/19720-8, 19/13569-8, 21/09952-0)

DIAS, LUCAS D.; BLANCO, KATE C.; DE FARIA, CLARA M. G.; DOZZA, CRISTINE; ZANCHIN, ELISSANDRA M.; PAOLILLO, FERNANDA R.; ZAMPIERI, KELY; LAURENTI, KAREN C.; SOUZA, KARINA J. O.; BRUNO, JULIANA S. A.; et al. Perspectives on photobiomodulation and combined light-based therapies for rehabilitation of patients after COVID-19 recovery. LASER PHYSICS LETTERS, v. 19, n. 4, p. 9-pg., 2022-04-01. (13/07276-1, 14/50857-8, 19/13569-8, 19/12694-3)

BERTOLO, MIRELLA ROMANELLI VICENTE; DIAS, LUCAS DANILO; FILHO, JOSEMAR GONCALVES DE OLIVEIRA; ALVES, FERNANDA; MARANGON, CRISIANE APARECIDA; AMARO MARTINS, VIRGINIA DA CONCEICA; FERREIRA, MARCOS DAVID; BAGNATO, VANDERLEI SALVADOR; PLEPIS, ANA MARIA DE GUZZI; BOGUSTZ JUNIOR, STANISLAU. Central composite design optimization of active and physical properties of food packaging films based on chitosan/gelatin/pomegranate peel extract. FOOD PACKAGING AND SHELF LIFE, v. 34, p. 11-pg., 2022-11-14. (13/07276-1, 14/50857-8, 19/13569-8, 18/22214-6, 18/24612-9, 19/22799-7, 19/18748-8)

RODRIGUES, FABIO M. S.; TAVARES, IURI; AROSO, RAFAEL T.; DIAS, LUCAS D.; DOMINGOS, CAROLINA V.; DE FARIA, CLARA M. G.; PICCIRILLO, GIUSI; MARIA, TERESA M. R.; CARRILHO, RUI M. B.; BAGNATO, VANDERLEI S.; et al. Photoantibacterial Poly(vinyl)chloride Films Applying Curcumin Derivatives as Bio-Based Plasticizers and Photosensitizers. Molecules, v. 28, n. 5, p. 16-pg., 2023-03-01. (18/00106-7, 19/13569-8, 13/07276-1, 14/50857-8)

LIMA, ALESSANDRA R.; DIAS, LUCAS D.; GARBUIO, MATHEUS; INADA, NATALIA M.; BAGNATO, VANDERLEI S.. A look at photodynamic inactivation as a tool for pests and vector-borne diseases control. LASER PHYSICS LETTERS, v. 19, n. 2, FEB 1 2022. (19/13569-8, 14/50857-8, 13/07276-1)

GARBUIO, MATHEUS; DIAS, LUCAS D.; DE SOUZA, LARISSA M.; CORREA, THAILA Q.; MEZZACAPPO, NATASHA F.; BLANCO, KATE C.; DE OLIVEIRA, KLEBER T.; INADA, NATALIA M.; BAGNATO, VANDERLEI S.. ormulations of curcumin and d-mannitol as a photolarvicide against Aedes aegypti larvae: Sublethal photolarvicidal action, toxicity, residual evaluation, and small-scale field trial{*. Photodiagnosis and Photodynamic Therapy, v. 38, JUN 2022. (19/27176-8, 14/50857-8, 20/06874-6, 19/13569-8, 13/07276-1)

SOUSA, JOANA F. M.; PINA, JOAO; GOMES, CARLA; DIAS, LUCAS D.; PEREIRA, MARIETTE M.; MURTINHO, DINA; DIAS, PAULA; AZEVEDO, JOAO; MENDES, ADELIO; MELO, J. SERGIO SEIXAS DE; et al. Transport and photophysical studies on porphyrin-containing sulfonated poly(etheretherketone) composite membranes. MATERIALS TODAY COMMUNICATIONS, v. 29, DEC 2021. (19/13569-8)

ALVES, FERNANDA; STRINGASCI, MIRIAN D.; REQUENA, MICHELLE B.; BLANCO, KATE C.; DIAS, LUCAS D.; CORREA, THAILA Q.; BAGNATO, VANDERLEI S.. Randomized and Controlled Clinical Studies on Antibacterial Photodynamic Therapy: An Overview. PHOTONICS, v. 9, n. 5, p. 36-pg., 2022-05-01. (21/09952-0, 19/12694-3, 13/07276-1, 19/13569-8, 21/01324-0)

AROSO, RAFAEL T.; DIAS, LUCAS D.; BLANCO, KATE C.; SOARES, JENNIFER M.; ALVES, FERNANDA; DA SILVA, GABRIELA J.; ARNAUT, LUIS G.; BAGNATO, VANDERLEI S.; PEREIRA, MARIETTE M.. Synergic dual phototherapy: Cationic imidazolyl photosensitizers and ciprofloxacin for eradication of in vitro and in vivo E. coli infections. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 233, p. 11-pg., 2022-06-09. (14/50857-8, 18/07961-0, 19/13569-8, 13/07276-1)

DIAS, LUCAS D.; BERTOLO, MIRELLA ROMANELLI, V; ALVES, FERNANDA; DE FARIA, CLARA M. G.; RODRIGUES, MURILO ALISON, V; LOPES, LETICIA KELLER B. C.; DE GUZZI PLEPIS, ANA MARIA; MATTOSO, LUIZ HENRIQUE C.; BOGUSZ JUNIOR, STANISLAU; BAGNATO, VANDERLEI S.. Preparation and characterization of curcumin and pomegranate peel extract chitosan/gelatin-based Films and their photoinactivation of bacteria. MATERIALS TODAY COMMUNICATIONS, v. 31, p. 10-pg., 2022-06-01. (19/18748-8, 14/50857-8, 19/13569-8)

BORGES, I. D.; DANIELLI, V, J. A.; SILVA, V. E. G.; SALLUM, L. O.; DE QUEIROZ, J. E.; DIAS, L. D.; IERMAK, I.; AQUINO, G. L. B.; CAMARGO, A. J.; VALVERDE, C.; et al. Synthesis and structural studies on (E)-3-(2,6-difluorophenyl)-1-(4-fluorophenyl)prop-2-en-1-one: a promising nonlinear optical material. RSC ADVANCES, v. 10, n. 38, p. 22542-22555, JUN 14 2020. (19/13569-8, 13/07276-1, 14/50857-8)

DIAS, LUCAS D.; ALVES, FERNANDA; BUZZA, HILDE H.; BAGNATO, VANDERLEI S.. Photodisinfection of material surfaces and bacterial skin infections by a detergent loaded with curcumin. Photodiagnosis and Photodynamic Therapy, v. 39, p. 7-pg., 2022-09-01. (18/00106-7, 19/13569-8, 14/50857-8, 21/01324-0, 13/07276-1)

ZANGIROLAMI, AMANDA C.; DIAS, LUCAS D.; BLANCO, KATE C.; VINAGREIRO, CAROLINA S.; INADA, NATALIA M.; ARNAUT, LUIS G.; PEREIRA, MARIETTE M.; BAGNATO, VANDERLEI S.. Avoiding ventilator-associated pneumonia: Curcumin-functionalized endotracheal tube and photodynamic action. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v. 117, n. 37, p. 22967-22973, SEP 15 2020. (13/07276-1, 14/50857-8, 19/13569-8, 19/12694-3)

MEZZACAPPO, NATASHA; DE SOUZA, LARISSA; INADA, NATALIA; DIAS, LUCAS; GARBUIO, MATHEUS; VENTURINI, FRANCINE; CORREA, THAILA; MOURA, LIDIA; BLANCO, KATE; DE OLIVEIRA, KLEBER; et al. Curcumin/d-mannitol as photolarvicide: induced delay in larval development time, changes in sex ratio and reduced longevity of Aedes aegypti. Pest Management Science, v. 77, n. 5, FEB 2021. (19/27176-8, 13/07276-1, 14/50857-8, 19/13569-8)

GONZALEZ, ANDREIA C. S.; DAMAS, LILIANA; AROSO, RAFAEL T.; TOME, VANESSA A.; DIAS, LUCAS D.; PINA, JOAO; CARRILHO, RUI M. B.; PEREIRA, MARLETTE M.. Monoterpene-based metallophthalocyanines: Sustainable synthetic approaches and photophysical studies. JOURNAL OF PORPHYRINS AND PHTHALOCYANINES, v. 24, n. 5-7, p. 947-958, MAY-JUL 2020. (19/13569-8)

MELO, NICOLAS JUNHITI DE; TOVAR, JOHAN S. D.; DOVIGO, LIVIA NORDI; DIAS, LUCAS D.; BAGNATO, VANDERLEI SALVADOR; INADA, NATALIA MAYUMI. Natural versus synthetic curcuminoids as photosensitizers: Photobleaching and antimicrobial photodynamic therapy evaluation. Photodiagnosis and Photodynamic Therapy, v. 42, p. 7-pg., 2023-03-28. (19/13569-8, 13/07276-1, 14/50857-8)

DIAS, LUCAS D.; CARBINATTO, FERNANDA MANSANO; ALMEIDA, ISABELLE DA SILVEIRA; BLANCO, KATE C.; MARQUELE-OLIVEIRA, FRANCIANE; MUNARI, CARLA CAROLINA; BAGNATO, VANDERLEI SALVADOR. Eco-Friendly Extraction of Green Coffee Oil for Industrial Applications: Its Antioxidant, Cytotoxic, Clonogenic, and Wound Healing Properties. FERMENTATION-BASEL, v. 9, n. 4, p. 13-pg., 2023-04-01. (18/00106-7, 19/13569-8, 13/07276-1, 14/50857-8)