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Synthesis of controlled hybrid materials: development of a bionanoplasmonic catalyst in light-driven one-pot cascade reactions

Grant number: 19/19551-3
Support type:Scholarships abroad - Research Internship - Scientific Initiation
Effective date (Start): December 01, 2019
Effective date (End): March 31, 2020
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Heloise Ribeiro de Barros
Grantee:Livia Yukari Tanaka
Supervisor abroad: Fernando Lopez Gallego
Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Local de pesquisa : Centre for Cooperative Research in Biomaterials (CIC biomaGUNE), Spain  
Associated to the scholarship:18/02435-8 - Synthesis of controlled hybrid materials based on Fe3O4 for catalytic applications, BP.IC

Abstract

The association of biocatalysts and nanocatalysts has been used as a remarkable approach for heterogeneous catalysis. Moreover, combining the catalytic activity of both noble metal nanoparticles and enzymes represents a potential breakthrough towards cascade reactions. Nevertheless, tailoring the optimal condition to ensure the synergic interaction over the different catalysts is still challenging. Therefore, the objective of this project is to develop a robust nanomaterial with bi-functional activity resulting from the combination of the properties of enzymes and plasmonic nanoparticles. As a proof-of-concept, gold nanoparticles (AuNPs) will be functionalized with lipase B from Candida antarctica (CALB) as a model bionanoplasmonic system. Different strategies of enzyme immobilization will be studied to elucidate the catalytic performance of CALB-AuNP system. Moreover, amino-functionalized Fe3O4@SiO2 will be used as support for CALB-AuNP in order to improve stability and to facilitate the recovery of the nanomaterial. We expect to obtain a bionanoplasmonic catalyst exhibiting stimulus-responsive activity upon light stimuli. The reaction kinetics will be studied by hydrolysis of p-nitrophenyl palmitate (pNPP) to p-nitrophenol (pNP) biocatalyzed by CALB. Subsequently, the following reduction of pNP to p-aminophenol (pAP) mediated by AuNPs will be investigated. Light irradiation will be used to explore the effect of localized surface plasmon resonance (LSPR) excitation of AuNPs over cascade reaction kinetics. Additionally, this study represents an opportunity to design novel materials with selective control of catalytic activity upon external stimulus regulation. Finally, the development of bionanoplasmonic hybrid materials will enable the study of sophisticated multi-step reaction cascades for potential application in biotechnology and biocatalysis studies.