Functional analysis of genes potentially associated with resistance to the basidiomycete Moniliophthora perniciosa in tomato (Solanum lycopersicum) cv. 'Micro-Tom'

• Grant number: 18/18711-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2019
• End date: September 30, 2023
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Antonio Vargas de Oliveira Figueira
• Grantee: Daniele Paschoal
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated research grant: 16/10498-4 - Investigation of strategies of adaptation to the pathogenic life style of fungi from the Moniliophthora genus at various levels of biological organizations: species, biotypes, and geographic lineages, AP.TEM
• Associated scholarship(s): 21/11434-8 - Dissecting the molecular aspects of M. perniciosa infection: identification of pathogen apoplastic effectors and plant immune receptors, BE.EP.DR

Abstract

"Witches' Broom" is a major disease of cacao, limiting cacao production in South America. The basidiomycete Moniliophthora perniciosa, the causal agent, presents a hemibiotrophic lifestyle with a peculiar, extensive biotrophic phase, promoting typical symptoms of hypertrophic growth and proliferation of infected shoots. The tomato (Solanum lycopersicum) cv. 'Micro-Tom' (MT) is a suitable genetic model to study the pathogenic interaction with M. perniciosa S-biotype, exhibiting typical symptoms of the infection, besides the tomato intrinsic characteristics, such as availability of an optimized genetic transformation protocol, transposing difficulties in cacao analysis. The RNA-seq analysis of interaction of the S-biotype x MT interaction enabled the identification of defense response mechanisms to constrain the colonization by M. perniciosa. Nevertheless, the lack of information related to infection by M. perniciosa makes fundamental the implementation of a platform for gene functional analysis, confirming the activated mechanisms to search for pathogen resistance. Furthermore, the comparison of both compatible (S-biotype) or incompatible (C-biotype) interactions with MT may indicate which are the key-factors associated with fungal colonization. Thus, this work proposes a functional analysis of genes associated with defense mechanisms, resistance, containment of fungal penetration and phosphate starvation during S-biotype or C-biotype infection using MT as a model system. The first step consists in the comparison of gene expression during infection by both C-biotype contrasting isolates and one S-biotype isolate via RNA-seq. At the second step, genes identified in RNA-seq analysis from the S-biotype M. perniciosa x MT interaction and the genes identified in the first step of this work will be overexpressed and/or silenced in MT. The knowledge of the molecular mechanisms of defense in MT will be essential to the understanding of the pathosystem and the establishment of control strategies for the "Witches' Broom Disease". (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PASCHOAL, DANIELE; COSTA, JULIANA L.; DA SILVA, EDER M.; DA SILVA, FABIA B.; CAPELIN, DIOGO; OMETTO, VITOR; ARICETTI, JULIANA A.; CARVALHO, GABRIEL G.; PIMPINATO, RODRIGO F.; DE OLIVEIRA, RICARDO F.; et al. Infection by Moniliophthora perniciosa reprograms tomato Micro-Tom physiology, establishes a sink, and increases secondary cell wall synthesis. Journal of Experimental Botany, v. 73, n. 11, p. 20-pg., 2022-02-21. (13/04309-6, 17/17000-4, 16/10498-4, 16/10524-5, 18/18711-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PASCHOAL, Daniele. Dissecting host specificity in the pathogenesis of S- and C-biotype isolates of Moniliophthora perniciosa, the causal agent of cacao witches\' broom disease. 2024. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.