Calcium aluminum cement-based scaffolds, incorporated with mesenchimal cells, for bone regeneration

• Grant number: 19/20095-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2019
• End date: September 30, 2020
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Luana Marotta Reis de Vasconcellos
• Grantee: Letícia Adrielly Dias Grisante
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Calcium aluminate cement (CAC) as biomaterial has been evaluated in relation to its physical, mechanical and biocompatibility properties, and its unique hardening and microstructure characteristics make it a prominent biomaterial. Studies related to the application of these cements in orthopedics that allow bone neoformation due to its composition and coefficient of thermal expansion similar to human bone. The objective of this work will be to evaluate the potential bone regeneration of aluminum cement Scaffolds and its incorporated or unincorporated schedules with mesenchymal cells in bone defects performed in rat forums. In this in vivo study, 26 Wistar rats will be used, which will be subjected to an inspection of 4.0 mm bone defects in both femurs, which will be filled with Scaffolds Initially there will be 48 Scaffolds based on CAC and their agendas (zirconia, zinc oxide and tricalcium phosphate), by the polymer sponge replica technique. Later, half of these scaffolds will be incorporated into the mesenchymal cells, preventing the entry of rats for a period of 48 hours. The animals will be randomly divided into 5 groups according to the bone defect filling material. In 4 groups (n = 4), the animals will receive CAC-based scaffolds not incorporated with mesenchymal cells in the right side defects and scaffolds of the same materials embedded with mesenchymal cells in the left side defects. In one group (n = 3), bone defects in both femurs will be filled with clot. All animals will be euthanized after 4 weeks of surgical procedure. Histological and histomorphometric assays will be performed. The data will be statistically analyzed and allowed by normality test to apply appropriate statistical test. The significance level adopted will be the conventional value of 5%.