| Grant number: | 19/15212-0 |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| Start date: | October 01, 2019 |
| End date: | March 31, 2024 |
| Field of knowledge: | Biological Sciences - Genetics - Animal Genetics |
| Principal Investigator: | Maria Dulcetti Vibranovski |
| Grantee: | Camila Correia Avelino |
| Host Institution: | Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Associated research grant: | 15/20844-4 - The role of gametogenesis on the origin and evolution of new genes, AP.JP |
| Associated scholarship(s): | 22/09955-2 - Impact of chromosomal translocations between X-autosomal in Drosophila melanogaster meiotic male X inactivation, BE.EP.DD |
Abstract Meiotic Sex Chromosome Inactivation (MSCI) is a process of transcriptional silencing of the sex chromosomes in the early stages of meiosis in spermatogenesis. Scientists have investigated the existence and impact of MSCI on D. melanogaster and some aspects related to spermatogenesis raised a great debate about the existence of this mechanism. During the period of the master's degree, the candidate participated in a collaboration with the laboratory of Professor Brian Oliver (NIDDK - NIH), and they found by Single-Cell RNA-seq a reduction of the X and IV expression, the IV chromosome was previously described in the literature as an ancestral X chromosome. In addition, the candidate performed cytogenetic experiments (Double Immuno-FISH) that found evidence of a depletion of RNA polymerase II on X and IV chromosomes in the late stages of meiotic prophase I, compared to the autosomes. As the double Immuno-FISH is a qualitative result, it is not possible to verify if the same genes that suffered a reduction of expression are those that did not show binding with RNA polymerase II in the late phases of prophase I. In order to investigate how MSCI mechanism occurs in its ancestral aspect and analyze gene regulation in Drosophila, this project intends to use three approaches: Double Immuno-FISH, for qualitative verification of MSCI in D. melanogaster; ChIP-seq, as a quantitative verification of the phenomenon observed by the Immuno-FISH and finally the differential expression analyses of RNA-seq data of the three main phases of spermatogenesis (mitosis, meiosis and post meiosis) from three species of the genus Drosophila, in order to observe the MSCI panorama in different evolutionary stages. Therefore, it is expected that these results will contribute to the comprehension of the evolution and regulation of MSCI in Drosophila male germline. (AU) | |
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