Electrochemical sensors with a matrix containing graphene quantum dots or nanoribbons for the detection of biomarkers

• Grant number: 19/13514-9
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: February 01, 2020
• End date: June 30, 2024
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Osvaldo Novais de Oliveira Junior
• Grantee: Jose Luiz Bott Neto
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis, AP.TEM
• Associated scholarship(s): 22/00243-0 - Developing new components and processes in visible light activated biosensors, BE.EP.PD

Abstract

Electrochemical sensors have been studied for the diagnosis of negligible diseases and cancer, however there are still challenges for the commercial production of the devices. One of the possible improvements is in the choice of nanomaterials for the matrix on which the active layer is deposited. In this project, we will use graphene quantum dots (GQDs) or graphene nanoribbons (GRNs) in the matrix for the electrochemical detection of biomarkers, such as prostate specific antigen (PSA), carbohydrate antigen 15-3 (CA 15-3) and vitamin D. The GQDs and GNRs will be synthesized by the Top-Down method. The molecular architectures of the sensors will be investigated by various techniques, including X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), atomic force spectroscopy (AFM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The detection of the biomarkers will be done with electrochemical techniques. Another important objective of this project is to investigate the adsorption and oxidation/reduction mechanisms of the biomarkers, using in situ Fourier transform infrared spectroscopy (FTIR) and in situ frequency summation spectroscopy (SFG). It will be tried to correlate the structure and composition of the electrochemical sensors with their electrocatalytic activity, stability, selectivity and sensitivity.

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BOTT-NETO, JOSE L.; MARTINS, THIAGO S.; BUSCAGLIA, LORENZO A.; SANTIAGO, PATRICIA V. B.; FERNANDEZ, PABLO S.; MACHADO, SERGIO A. S.; OLIVEIRA JR, OSVALDO N.. A portable system for photoelectrochemical detection of lactate on TiO2 nanoparticles and [Ni(salen)] polymeric film. SENSORS AND ACTUATORS B-CHEMICAL, v. 345, OCT 15 2021. (18/22214-6, 19/00101-8, 17/11986-5, 19/13514-9, 18/20952-0)

SOARES, JULIANA COATRINI; SOARES, ANDREY COATRINI; RODRIGUES, VALQUIRIA CRUZ; OITICICA, PEDRO RAMON ALMEIDA; RAYMUNDO-PEREIRA, PAULO AUGUSTO; BOTT-NETO, JOSE LUIZ; BUSCAGLIA, LORENZO A.; DE CASTRO, LUCAS DANIEL CHIBA; RIBAS, LUCAS C.; SCABINI, LEONARDO; et al. Detection of a SARS-CoV-2 sequence with genosensors using data analysis based on information visualization and machine learning techniques. MATERIALS CHEMISTRY FRONTIERS, v. 5, n. 15, p. 5658-5670, AUG 7 2021. (19/07811-0, 18/19750-3, 20/02938-0, 16/01919-6, 18/18953-8, 19/13514-9, 14/50867-3, 16/23763-8, 18/22214-6, 19/00101-8)

MARTINS, THIAGO S.; BOTT-NETO, JOSE L.; OLIVEIRA, JR., OSVALDO N.; MACHADO, SERGIO A. S.. A sandwich-type electrochemical immunosensor based on Au-rGO composite for CA15-3 tumor marker detection. Microchimica Acta, v. 189, n. 1, JAN 2022. (18/22214-6, 19/13514-9, 19/01777-5)

MARTINS, THIAGO S.; BOTT-NETO, JOSE L.; OLIVEIRA, JR., OSVALDO N.; MACHADO, SERGIO A. S.. Paper-based electrochemical sensors with reduced graphene nanoribbons for simultaneous detection of sulfamethoxazole and trimethoprim in water samples. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 882, FEB 1 2021. (19/13514-9, 18/22214-6, 19/01777-5)

MARTINS, THIAGO S.; MACHADO, SERGIO A. S.; OLIVEIRA JR, OSVALDO N.; BOTT-NETO, JOSE L.. Optimized paper-based electrochemical sensors treated in acidic media to detect carbendazim on the skin of apple and cabbage. Food Chemistry, v. 410, p. 7-pg., 2023-01-13. (19/13514-9, 22/03758-0, 18/22214-6, 19/01777-5)

NETO, JOSE L. BOTT; MARTINS, THIAGO S.; MACHADO, SERGIO A. S.; OLIVEIRA JR, OSVALDO N.. Enhanced photocatalysis on graphitic carbon nitride sensitized with gold nanoparticles for photoelectrochemical immunosensors. Applied Surface Science, v. 606, p. 6-pg., 2022-12-30. (19/13514-9, 18/22214-6, 19/01777-5)

BOTT-NETO, JOSE L.; MARTINS, THIAGO S.; OLIVEIRA, OSVALDO N.; MARKEN, FRANK. Controlled electrodeposition of brookite TiO₂ for photoelectroanalysis at printed carbon electrodes. Applied Surface Science, v. 640, p. 7-pg., 2023-12-15. (19/13514-9, 18/22214-6, 22/03758-0, 22/00243-0)

SOARES, JULIANA COATRINI; SOARES, ANDREY COATRINI; RODRIGUES, VALQUIRIA CRUZ; OITICICA, PEDRO RAMON ALMEIDA; RAYMUNDO-PEREIRA, PAULO AUGUSTO; BOTT-NETO, JOSE LUIZ; BUSCAGLIA, LORENZO A.; DE CASTRO, LUCAS DANIEL CHIBA; RIBAS, LUCAS C.; SCABINI, LEONARDO; et al. Detection of a SARS-CoV-2 sequence with genosensors using data analysis based on information visualization and machine learning techniques. MATERIALS CHEMISTRY FRONTIERS, JUN 2021. (18/22214-6, 18/19750-3, 18/18953-8, 16/01919-6, 19/13514-9, 14/50867-3, 20/02938-0, 19/00101-8, 19/07811-0, 16/23763-8)

MARTINS, THIAGO S.; BOTT-NETO, JOSE L.; MACHADO, SERGIO A. S.; OLIVEIRA, OSVALDO N.. Label-Free Electrochemical Immunosensor Made with Tree-like Gold Dendrites for Monitoring 25-Hydroxyvitamin D3 Metabolite. ACS APPLIED MATERIALS & INTERFACES, v. 14, n. 27, p. 8-pg., 2022-07-13. (19/13514-9, 18/22214-6, 19/01777-5)

MARTINS, THIAGO S.; BOTT-NETO, JOSE L.; MACHADO, SERGIO A. S.; OLIVEIRA JR, OSVALDO N.. Label-Free Electrochemical Immunosensor Made with Tree-like Gold Dendrites for Monitoring 25-Hydroxyvitamin D3 Metabolite. ACS APPLIED MATERIALS & INTERFACES, v. N/A, p. 8-pg., 2022-07-01. (19/01777-5, 19/13514-9, 18/22214-6)

BOTT-NETO, JOSE L.; MARTINS, THIAGO S.; BUSCAGLIA, LORENZO A.; MACHADO, SERGIO A. S.; OLIVEIRA, OSVALDO N. JR JR. Photocatalysis of TiO2 Sensitized with Graphitic Carbon Nitride and Electrodeposited Aryl Diazonium on Screen-Printed Electrodes to Detect Prostate Specific Antigen under Visible Light. ACS APPLIED MATERIALS & INTERFACES, v. 14, n. 19, p. 8-pg., 2022-05-18. (19/13514-9, 19/00101-8, 18/22214-6, 19/01777-5)