Effect of calorie restriction on the structure of 20S proteasome: a cryo-EM study

• Grant number: 19/27044-4
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: February 28, 2020
• End date: July 17, 2020
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Fernanda Marques da Cunha
• Grantee: Renata Naporano Bicev
• Supervisor: Guillaume Tresset
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Institution abroad: Laboratoire de Physique des Solides (LPS), France
• Associated to the scholarship: 16/11724-8 - Calorie Restriction effects on the proteasome of yeast Saccharomyces cerevisiae, BP.DR

Abstract

Calorie restriction (CR) is a nutritional intervention that has been shown to extend lifespan in a variety species and also to afford metabolic benefits. The mechanisms involved in these CR benefits are not fully elucidated. Data from our group demonstrated that Saccharomyces cerevisiae cells cultured under calorie restricted conditions apart from having a life span extension, showed increased proteasome activity. This increase is not due to increased proteasome amount, suggesting that other mechanisms are involved in calorie restriction-induced increase in proteasome activity. The present BEPE project aims to characterize the effect of CR on proteasome spatial conformation, as allosteric regulation was shown to be a common control mechanism of proteasome activity. In order to perform this structural characterization, we are going to use cryo-EM. (AU)