Calorie restriction effects on the proteasome of yeast Saccharomyces Cerevisiae

• Grant number: 16/11724-8
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): October 01, 2016
• Effective date (End): June 30, 2020
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Fernanda Marques da Cunha
• Grantee: Renata Naporano Bicev
• Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated research grant: 13/07937-8 - Redoxome - Redox Processes in Biomedicine, AP.CEPID

Abstract

Calorie restriction (CR) is a nutritional intervention capable of affording a number of metabolic benefits to organisms that may lead to lifespan extension. In spite of being studied for more than 80 years, the mechanisms through which CR exerts its effects are not completely elucidated. Ubiquitin-proteasome system (UPS) is a proteolytic system through which intracellular proteins are degraded in a regulated manner. A number of studies in the literature indicate that UPS is crucial for cellular homeostasis. Data from our group demonstrate that Saccharomyces cerevisiae cells cultured under calorie restricted conditions have increased proteasome activity that is not due to increased proteasome amount. The present project aims to characterize the effect of CR on parameters that affect proteasome catalytic activity such as 19S/20S coupling, post translational modifications as well as tridimensional conformation. A detailed characterization of CR-induced modifications and understanding of its mechanisms of action will pave the way for the development of therapeutic strategies or identification of molecular targets to improve health.