Evaluation of the therapeutic efficacy of physical activity-associated nanoparticle-labeled stem cells in the focal ischemia model

• Grant number: 19/21070-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2020
• End date: December 31, 2020
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Lionel Fernel Gamarra Contreras
• Grantee: João Victor Matias Ferreira
• Host Institution: Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE). São Paulo , SP, Brazil

Abstract

The purpose of this Scientific Initiation project is to evaluate the efficiency of cell therapy combined with physical activity after induction of focal cerebral ischemia using the photothrombosis technique in the animal model. The present study is part of a larger project where we intend to study the cellular and molecular mechanisms in tissue and functional repair through the therapeutic action of bone marrow mesenchymal stem cells (MTC) labeled with multimodal nanoparticles in the animal model with focal ischemia. With the use of nanobiotechnological resources will be possible to monitor these cells in vivo by molecular imaging techniques, enabling the study of cell tracking and migration that will assist in the functional characterization of these cells in the photothrombosis-induced ischemic brain region of the experimental model.Thus, the present project of scientific initiation will be divided into four stages: the first stage will consist of a systematic review based on scientific articles to evaluate the parameters used in the induction of focal cerebral ischemia using the photothrombosis technique. In the second stage will be performed the marking of the MSC with multimodal nanoparticles. The third stage corresponds to the phototrmobesis stroke induction in the animal model. In the fourth stage implantation of the nanoparticle-labeled MSCs in the animal and subsequent evaluation of the MSM homing and tracking. Finally, in the fifth stage will be performed the behavioral assessment and motor activity.

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

THEINEL, MATHEUS H.; NUCCI, MARIANA P.; ALVES, ARIELLY H.; DIAS, OLIVIA F. M.; MAMANI, JAVIER B.; GARRIGOS, MURILO M.; OLIVEIRA, FERNANDO A.; REGO, GABRIEL N. A.; VALLE, NICOLE M. E.; CIANCIARULLO, GABRIELA; et al. The Effects of Omega-3 Polyunsaturated Fatty Acids on Breast Cancer as a Preventive Measure or as an Adjunct to Conventional Treatments. NUTRIENTS, v. 15, n. 6, p. 34-pg., 2023-03-01. (16/21470-3, 17/17868-4, 19/21070-3)

GARRIGOS, MURILO M.; OLIVEIRA, FERNANDO A.; NUCCI, MARIANA P.; MAMANI, JAVIER B.; DIAS, OLIVIA F. M.; REGO, GABRIEL N. A.; JUNQUEIRA, MARA S.; COSTA, CICERO J. S.; SILVA, LUCAS R. R.; ALVES, ARIELLY H.; et al. Bioluminescence Imaging and ICP-MS Associated with SPION as a Tool for Hematopoietic Stem and Progenitor Cells Homing and Engraftment Evaluation. PHARMACEUTICS, v. 15, n. 3, p. 18-pg., 2023-03-01. (16/21470-3, 17/17868-4, 19/21070-3)

NUCCI, MARIANA P.; OLIVEIRA, FERNANDO A.; FERREIRA, JOAO M.; PINTO, YOLANDA O.; ALVES, ARIELLY H.; MAMANI, JAVIER B.; NUCCI, LEOPOLDO P.; VALLE, NICOLE M. E.; GAMARRA, LIONEL F.. ffect of Cell Therapy and Exercise Training in a Stroke Model, Considering the Cell Track by Molecular Image and Behavioral Analysi. CELLS, v. 11, n. 3, FEB 2022. (16/21470-3, 19/21070-3, 17/17868-4)

GARRIGOS, MURILO MONTENEGRO; DE OLIVEIRA, FERNANDO ANSELMO; NUCCI, MARIANA PENTEADO; NUCCI, LEOPOLDO PENTEADO; ALVES, ARIELLY DA HORA; METROPOLO DIAS, OLIVIA FURIAMA; GAMARRA, LIONEL FERNEL. How mesenchymal stem cell cotransplantation with hematopoietic stem cells can improve engraftment in animal models. WORLD JOURNAL OF STEM CELLS, v. 14, n. 8, p. 22-pg., 2022-08-26. (16/21470-3, 17/17868-4, 19/21070-3)

MURILO MONTENEGRO GARRIGÓS; FERNANDO ANSELMO DE OLIVEIRA; CÍCERO JÚLIO SILVA COSTA; LUCAS RENAN RODRIGUES; MARIANA PENTEADO NUCCI; ARIELLY DA HORA ALVES; JAVIER BUSTAMANTE MAMANI; GABRIEL NERY DE ALBUQUERQUE REGO; JUAN MATHEUS MUNOZ; LIONEL FERNEL GAMARRA. Assessing the toxicity of one-step-synthesized PEG-coated gold nanoparticles: in vitro and in vivo studies. Einstein (São Paulo), v. 22, 2024-05-20. (16/21470-3, 17/17868-4, 19/21070-3)

DIAS, OLIVIA FURIAMA METROPOLO; DO VALLE, NICOLE MASTANDREA ENNES; DE OLIVEIRA, FERNANDO ANSELMO; ALVES, ARIELLY DA HORA; MAMAMI, JAVIER BUSTAMANTE; REGO, GABRIEL NERY DE ALBUQUERQUE; DA SILVA, KEITHY FELIX; GALANCIAK, MARTA CAETANO DOS SANTOS; MUNOZ, JUAN MATHEUS; CINTRA, LUCIANA; et al. Assessing the impact of sex and circadian rhythms on rodent behavior: refining preclinical study designs. Einstein (São Paulo), v. 23, p. 13-pg., 2025-01-01. (17/17868-4, 19/21070-3, 24/03247-1, 23/10843-7)

MUNOZ, JUAN MATHEUS; PILEGGI, GIOVANA FONTANELLA; NUCCI, MARIANA PENTEADO; ALVES, ARIELLY DA HORA; PEDRINI, FLAVIA; DO VALLE, NICOLE MASTANDREA ENNES; MAMANI, JAVIER BUSTAMANTE; DE OLIVEIRA, FERNANDO ANSELMO; LOPES, ALEXANDRE TAVARES; CARRENO, MARCELO NELSON PAEZ; et al. In Silico Approach to Model Heat Distribution of Magnetic Hyperthermia in the Tumoral and Healthy Vascular Network Using Tumor-on-a-Chip to Evaluate Effective Therapy. PHARMACEUTICS, v. 16, n. 9, p. 21-pg., 2024-09-01. (16/21470-3, 24/03247-1, 19/21070-3, 17/17868-4)

ALVES, ARIELLY DA HORA; ENNES DO VALLE, NICOLE MASTANDREA; YOKOTA-MORENO, BRUNO YUKIO; GALANCIAK, MARTA CAETANO DOS SANTOS; FELIX DA SILVA, KEITHY; MAMANI, JAVIER BUSTAMANTE; SERTIE, ANDREA LAURATO; DE OLIVEIRA, FERNANDO ANSELMO; NUCCI, MARIANA PENTEADO; GAMARRA, LIONEL FERNEL. Stem cell-derived neural organoids as platforms to investigate glioblastoma invasion and migration: A systematic review. WORLD JOURNAL OF STEM CELLS, v. 17, n. 8, p. 24-pg., 2025-08-26. (19/21070-3, 23/10843-7)

NUCCI, MARIANA P.; MAMANI, JAVIER B.; OLIVEIRA, FERNANDO A.; FILGUEIRAS, IGOR S.; ALVES, ARIELLY H.; THEINEL, MATHEUS H.; RODRIGUES, LUIZ D.; MARTI, LUCIANA; GAMARRA, LIONEL F.. Optimization of Multimodal Nanoparticles Internalization Process in Mesenchymal Stem Cells for Cell Therapy Studies. PHARMACEUTICS, v. 14, n. 6, p. 23-pg., 2022-06-01. (19/21070-3, 16/21470-3, 17/17868-4)

ALVES, ARIELLY DA HORA; NUCCI, MARIANA PENTEADO; DO VALLE, NICOLE MASTANDREA ENNES; MISSINA, JULIANA MORAIS; MAMANI, JAVIER BUSTAMANTE; REGO, GABRIEL NERY ALBUQUERQUE; DIAS, OLIVIA FURIAMA METROPOLO; GARRIGOS, MURILO MONTENEGRO; DE OLIVEIRA, FERNANDO ANSELMO; GAMARRA, LIONEL FERNEL. Current overview of induced pluripotent stem cell-based blood-brain barrier-on-a-chip. WORLD JOURNAL OF STEM CELLS, v. 15, n. 6, p. 22-pg., 2023-06-26. (16/21470-3, 17/17868-4, 19/21070-3)

VALLE, NICOLE M. E.; NUCCI, MARIANA P.; ALVES, ARIELLY H.; RODRIGUES, LUIZ D.; MAMANI, JAVIER B.; OLIVEIRA, FERNANDO A.; LOPES, CAIQUE S.; LOPES, ALEXANDRE T.; CARRENO, MARCELO N. P.; GAMARRA, LIONEL F.. Advances in Concentration Gradient Generation Approaches in a Microfluidic Device for Toxicity Analysis. CELLS, v. 11, n. 19, p. 56-pg., 2022-10-01. (16/21470-3, 17/17868-4, 19/21070-3)