Physicochemical and biological characteristics of clinker and cements MTA and MTA HP repair Angelus

• Grant number: 19/24837-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2020
• End date: July 31, 2021
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Mario Tanomaru Filho
• Grantee: Bruna Vasconcellos de Oliveira
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

New calcium silicate-based cement formulations are developed. Currently, MTA Angelus is composed of calcium silicate (clinker) plus calcium tungstate, being manipulated with water (MTA or liquid with additives (MTAHP Repair). The aim of this study will be to evaluate the effect of radiopacifier and liquid on the properties of MTA Angelus. The physicochemical properties, viability and cellular bioactivity of clinker Angelus, MTA and MTAHP will be evaluated. The setting time, radiopacity and solubility tests will be based on ISO 6876. The pH measurement will be performed by a digital pH meter. MTT and neutral red will be performed to define the eluate dilution of the materials, and a new MTT assay and alkaline phosphatase assay will be performed to assess cell viability and bioactivity after contact with eluted materials for 3 and 7 days. Alizarin will be performed to identify the calcium deposit in cell culture after 21 days of contact with the cement. The data obtained for each evaluation will be submitted to the appropriate statistical tests, with 5% significance level. (AU)