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Characterization of virulence factors and resistance in Staphylococcus aureus isolated from children and adolescents living in rural settlements

Grant number: 19/21469-3
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): March 01, 2020
Effective date (End): December 31, 2020
Field of knowledge:Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
Principal Investigator:Valéria Cataneli Pereira
Grantee:Maria Clara Antunes Alves
Home Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil

Abstract

Staphylococcus aureus is a human commensal bacteria that can cause a number of diseases. Being a nasal carrier of S. aureus, the individual may develop the characteristic signs and symptoms of infection, or remain asymptomatic. Virulence factors such as the production of biofilms, staphylococcal enterotoxins and Leucocidine Panton Valentine favor the permanence, invasion and dissemination of these strains in the community. Thus this study aims to characterize the virulence factors of S. aureus isolated children and adolescents living in a rural settlement of Pontal do Paranapanema. It will be studied isolated S. aureus 80 individuals under 18 years old who live in rural settlement and 30 individuals in the same age group who have no contact with the settlements. After isolation of S. aureus will be performed DNA extraction and detection of biofilm genes, classic staphylococcal enterotoxins, Leucocidine Panton Valentine by Polymerase Chain Reaction (PCR). For analysis of the obtained results will be used the X2 Test. The significance level for all tests will be set at p <0.05. (AU)