Proteomic profile of endodontic infections in diabetic patients

• Grant number: 19/14995-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2020
• End date: October 31, 2022
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Rogerio de Castilho Jacinto
• Grantee: Caroline Loureiro
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Chronic endodontic infections are more prevalent in diabetic patients when compared to healthy patients, suggesting that Diabetes Mellitus may be a modifying factor for endodontic diseases. Endodontic infections are caused by a multi-species community of bacteria. As the methods of microbiological identification and quantification of endotoxins, are not able to infer the physiology, function and microbial pathogenicity, proteomic analysis is a technique that may contribute to the study of the pathogenesis of endodontic infections and the pathogen/host relationship. The present study aims: to qualitatively and quantitatively analyze the proteomic profile of endodontic infections in diabetic patients compared to systemically healthy patients; to correlate the expression of the bacterial proteins detected in the root canals and their biological functions in the two study groups. Samples will be collected from 18 patients referred for root canal treatment at the Faculty of Dentistry of Araçatuba FOA - UNESP, divided into two groups: control - systemically healthy patients (n = 9) and diabetic patients (n = 9). The sample will include asymptomatic teeth with primary endodontic infection, and presence of periapical lesion visible on radiography (chronic apical periodontitis). The identification of the peptides will be done in a nanoACQUITY UPLC-Xevo QTof MS system (Waters) using ProteinLynx Global Server (PLGS) software version 3.0, using the UniprotKB database (http://www.uniprot.org). The difference in protein expression will be statistically analyzed with Monte Carlo algorithm incorporated into the software (p <0.05). (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOUREIRO, C.; BUZALAF, M. A. R.; MORAES, F. R. N.; VENTURA, T. M. O.; PELA, V. T.; PESSAN, J. P.; JACINTO, R. C.. Quantitative proteomic analysis in symptomatic and asymptomatic apical periodontitis. International Endodontic Journal, FEB 2021. (19/14995-0, 18/08282-9, 18/18741-0)

LOUREIRO, CAROLINE; BUZALAF, MARILIA AFONSO RABELO; VENTURA, TALITA MENDES OLIVEIRA; PELA, VINICIUS TAIOQUI; RODRIGUES, GLADISTON WILLIAM LOBO; ANDRADE, JULIA GUERRA; PESSAN, JULIANO PELIM; JACINTO, ROGERIO CASTILHO. Teeth with acute apical abscess vs. teeth with chronic apical periodontitis: a quantitative and qualitative proteomic analysis. CLINICAL ORAL INVESTIGATIONS, v. 27, n. 2, p. 11-pg., 2022-11-29. (19/14995-0, 18/18741-0)

LOUREIRO, CAROLINE; BUZALAF, MARILIA AFONSO RABELO; PESSAN, JULIANO PELIM; VENTURA, TALITA MENDES OLIVEIRA; PELA, VINICIUS TAIOQUI; RIBEIRO, ANA PAULA FERNANDES; JACINTO, ROGERIO DE CASTILHO. Proteomic analysis of infected root canals with apical periodontitis in patients with type 2 diabetes mellitus: A cross-sectional study. International Endodontic Journal, v. 55, n. 9, p. 13-pg., 2022-07-14. (19/14995-0, 18/18741-0)

CAROLINE LOUREIRO; MARÍLIA AFONSO RABELO BUZALAF; JULIANO PELIM PESSAN; FELIPE RICARDO NUNES DE MORAES; VINÍCIUS TAIOQUI PELÁ; TALITA MENDES OLIVEIRA VENTURA; ROGÉRIO DE CASTILHO JACINTO. Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study. Brazilian Dental Journal, v. 31, n. 3, p. 319-336, 2020-07-13. (18/08282-9, 18/18741-0, 19/14995-0)

LOUREIRO, C.; BUZALAF, M. A. R.; MORAES, F. R. N.; VENTURA, T. M. O.; PELA, V. T.; PESSAN, J. P.; JACINTO, R. C.. Quantitative proteomic analysis in symptomatic and asymptomatic apical periodontitis. International Endodontic Journal, v. 54, n. 6, p. 14-pg., 2021-02-17. (18/08282-9, 19/14995-0, 18/18741-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LOUREIRO, Caroline. Proteomic profile of endodontic infections in diabetic patients. 2023. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.