Structural studies of proteins incorporated in ordered mesoporous silica

• Grant number: 19/19567-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2020
• End date: February 28, 2022
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Jose Luiz de Souza Lopes
• Grantee: Jéssica Aparecida Ferreira Pedro
• Host Institution: Instituto de Física (IF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 17/17844-8 - Nanostructured silica as a protective vehicle for vaccines and biomolecules, AP.TEM

Abstract

Ordered mesoporous silicas are excellent carriers for protein encapsulation because they have pores of different diameters. During the process of protein adsorption on the silica surface, distinct intermolecular interactions are established which can provide the proteins greater structural/functional stability in chemical, mechanical and thermal treatments. SBA-15 is one of the most studied ordered mesoporous silica particles, whose action as an antigen protective vehicle in vaccines is already known. In this work, we propose the study of incorporation of proteins in SBA-15 with surface containing different functional groups (hydrophilic, hydrophobic). The possible structural changes in proteins mainly composed of distinct elements of secondary structure will be investigated, as well as the influence of different functional groups on the surface of the SBA-15 during the process of protein adsorption and their release in buffer solutions and in solutions that mimic the gastrointestinal fluid will be monitored. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PEDRO, Jéssica Aparecida Ferreira. Structural studies of proteins incorporated in ordered mesoporous silica. 2022. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Física (IF/SBI) São Paulo.