Structural proteomics: design, synthesis and application of new generation crosslinking agents

• Grant number: 20/00144-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: July 01, 2020
• End date: June 30, 2023
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Fabio Cesar Gozzo
• Grantee: Rafael Douglas Clemente Gallo
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 14/17264-3 - New frontiers in structural proteomics: characterizing protein and protein complex structures by mass spectrometry, AP.TEM

Abstract

The structure determination of proteins and their interactions with other proteins are key informations in the elucidation of the mechanism of action of these macromolecules. The combinationof cross-linking with Mass Spectrometry (MS) is a strategy that allows the structural characterization of proteins and protein complexes, especially when high resolution techniques cannot be employed. Mass spectrometry analysis can be considered a universal technique, which can be applied to a large variety of proteins. In addition, this strategy benefits from all the advantages of MS, such as high sensibility, fast analysis and ease of use. Although many protein-protein complexes have been already successfully resolved, there are limitations yet to be addressed, in order to make the cross-linking/MS a more general strategy. Among the greatest challenges in the field, the detection and identification of cross-linked species is of special importance. The current project aims at the development of new Cross-Linking Agents (CLAs) for resolving the problems mentioned above, therefore allowing the enrichment of the number of modified species, thus overcoming their challenging detection. The new CLAs are expected to facilitate the identification of these species due to the use of markers containing mass defects, which should allow a clear distinction between modified and non-modified species. The new families of CLAs proposed here have the potential to generate a great advancement on the quality of the data obtained, and significantly increase the applicability of this technique. (AU)

Scientific publications

(7)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

MUNARETTO, LAIELI S.; DOS SANTOS, CAIO Y.; GALLO, RAFAEL D. C.; OKADA JR, CELSO Y.; DEFLON, VICTOR M.; JURBERG, IGOR D.. Visible-Light-Mediated Strategies to Assemble Alkyl 2-Carboxylate-2,3,3-Trisubstituted beta-Lactams and 5-Alkoxy-2,2,4-Trisubstituted Furan-3(2H)-ones Using Aryldiazoacetates and Aryldiazoketones. ORGANIC LETTERS, v. 23, n. 23, p. 9292-9296, DEC 3 2021. (19/01235-8, 20/00144-6)

GALLO, RAFAEL D. C.; DUARTE, MARCELO; DA SILVA, AMANDA F.; OKADA, JR., CELSO Y.; DEFLON, VICTOR M.; JURBERG, IGOR D.. Selective C-C Bond Cleavage Strategy Promoted by Visible Ligh. ORGANIC LETTERS, v. 23, n. 22, p. 8916-8920, NOV 19 2021. (20/00144-6, 19/01235-8)

STIVANIN, MATEUS L.; GALLO, RAFAEL D. C.; SPADETO, JOAO PAULO M.; CORMANICH, RODRIGO A.; JURBERG, IGOR D.. visible light-mediated three-component strategy based on the ring-opening of cyclic ethers with aryldiazoacetates and nucleophile. ORGANIC CHEMISTRY FRONTIERS, v. 9, n. 5, p. 1321-1326, MAR 1 2022. (20/00144-6, 20/06536-3, 18/03910-1, 19/01235-8)

GALLO, RAFAEL D. C.; CARIELLO, GUILHERME; GOULART, TALES A. C.; JURBERG, IGOR D.. Visible light-mediated photolysis of organic molecules: the case study of diazo compounds. CHEMICAL COMMUNICATIONS, v. 59, n. 48, p. 15-pg., 2023-05-11. (22/01104-3, 20/00144-6, 22/01750-2, 19/17721-9)

CARIELLO, GUILHERME; GALLO, RAFAEL D. C.; DEFLON, VICTOR M.; CORMANICH, RODRIGO A.; JURBERG, IGOR D.. Visible light-mediated formal alkylation and [4+1]-cycloaddition strategies of silyl enol ethers with aryldiazoacetates. CHEMICAL COMMUNICATIONS, v. 61, n. 10, p. 5-pg., 2024-12-03. (22/01104-3, 22/16188-8, 20/00144-6, 18/03910-1)

MUNARETTO, LAIELI S.; GALLO, RAFAEL D. C.; LEAO, LUIZ PAULO M. O.; JURBERG, IGOR D.. H-F bond insertions into alpha-diazo carbonyl compounds. ORGANIC & BIOMOLECULAR CHEMISTRY, v. 20, n. 31, p. 5-pg., 2022-03-24. (20/00144-6, 19/01235-8)

CAPELLARO, KAUE C.; GOULART, TALES A. C.; GALLO, RAFAEL D. C.; DE S. SCHENFEL, JULIANA; JURBERG, IGOR D.. Friedel-Crafts-Type Arylation Strategy for the Conversion of Alkyl 2-((Diphenoxyphosphoryl)oxy)-2-arylacetates to α,α-Diaryl Esters. ACS ORGANIC & INORGANIC AU, v. 4, n. 1, p. 7-pg., 2023-11-09. (19/09380-7, 20/00144-6, 22/01750-2, 22/01104-3, 19/17721-9)