Role of Angiotensin II on the in vitro osteoblastic differentiation of SPONTANEALLY HYPERTENSE mice in bone marrow stromal mesenchymal cells

• Grant number: 19/26149-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2020
• End date: February 28, 2021
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Sandra Helena Penha de Oliveira
• Grantee: Ana Paula Moura Leal
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated research grant: 15/03965-2 - Role of the renin-angiotensin system in different oral inflammatory models: an experimental interdisciplinary and clinical approach, AP.TEM

Abstract

Arterial hypertension and osteoporosis represent multifactorial disorders, where genetic and environmental factors culminate in the development of pathogenesis. Recent studies suggest that angiotensin II, the main active peptide produced by the renin-angiotensin system (RAS), stimulates osteoclast differentiation and activity, influencing bone metabolism and contributing to the imbalance between bone formation and resorption. Based on this principle, this work aims to evaluate the role of AT1 and AT2 receptors on the osteoblastic differentiation induced by angiotensin II in cultures of bone marrow stromal mesenchymal stem cells (CTM-MO) of spontaneously hypertensive animals (SHR). For that, adult SHR rats will be used where we will evaluate parameters: cell proliferation; total protein, alkaline phosphatase activity; and formation of a mineralized matrix of osteoblasts stimulated by angiotensin II in the presence of AT1 or AT2 receptor antagonists.