Analysis of satelitomas of different populations of Astyanax lacustris

• Grant number: 19/24914-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2020
• End date: December 31, 2021
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Fábio Porto-Foresti
• Grantee: Rodrigo Milan Calegari
• Host Institution: Faculdade de Ciências (FC). Universidade Estadual Paulista (UNESP). Campus de Bauru. Bauru , SP, Brazil

Abstract

The study of repeat sequences was greatly impacted by the popularization of Next Generation Sequencing, along with developing new scripts and softwares. With these, bioinformatic collections, such as RepeatExplorer and SatMiner, have been reducing the price and the time of analysis, allowing a higher throughput of the analyzes. Satellite DNA (satDNAs) are repeated tandem sequences and the complete catalog of these sequences in a particular specie is called satellitome. Generally, satDNAs don't code any final product, and their function is often totally unknown in the genome. In this sense, this sequences are subject to rapid evolution, making satelitomes of nearby species quite divergent. In this context this study aims to characterize and compare the satellitome of different populations of Astyanax lacustris, using tools developed with the support of bioinformatics, in order to better understand the evolutionary processes that they are subject to repetitive sequences of satellite DNA in the genome of the same species, found in different basins.